iFluor488-dUTP：更亮的信號，更強的光穩定性

染料修飾的脫氧尿苷5'-三磷酸鹽是通過常規酶摻入方法（如逆轉錄、缺口翻譯、隨機引物標記或PCR）生產染料標記DNA的最常見方法之一。這種酶熒光標記方法廣泛用于FISH探針和基于微陣列的實驗。這種iFluor 488 dUTP綴合物使用SpectrumGreen?濾光片組作為綠色熒光色（SpectrumGreen?是Vysis的商標）。與常用的綠色探針（如熒光素-12-dUTP）相比，iFluor 488提供了更亮的信號，具有更強的光穩定性，不受pH值的影響。

艾美捷iFluor488-dUTP：

貨號：AAT-17044

單位大小：25 納摩爾

分子量：1152.83

溶劑：水

校正因子（260 納米）：0.21

校正因子（280 納米）：0.11

消光系數（cm -1 M -1）：75000

激發（納米）：491

發射（納米）：516

量子產率：0.9

H-短語：H303, H313, H333

危險符號：XN

預期用途：僅限研究使用（RUO）

R-短語：R20, R21, R22

儲存：冷凍（< -15 °C）；盡量減少光照暴露

圖：熒光素和iFluor?488 dUTP標記的端粒探針在中期HeLa細胞中的熒光原位雜交。

iFluor488-dUTP文獻參考：

Anti-proliferative effect of Fe(III) complexed with 1-(2-hydroxy-3-methoxybenzaldehyde)-4-aminosalicylhydrazone in HepG2 cells.

Authors: Fukushima, Takeshi and Taniguchi, Erina and Yamada, Hiroshi and Kato, Kiyomasa and Shimizu, Ayako and Nishiguchi, Yoshikazu and Onozato, Mayu and Ichiba, Hideaki and Azuma, Yutaro

Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2015): 669-77

Localisation of abundant and organ-specific genes expressed in Rosa hybrida leaves and flower buds by direct in situ RT-PCR.

Authors: Jedrzejuk, Agata and Mibus, Heiko and Serek, Margrethe

Journal: TheScientificWorldJournal (2012): 609597

SNP genotyping using microsphere-linked PNA and flow cytometric detection.

Authors: Rockenbauer, Eszter and Petersen, Kenneth and Vogel, Ulla and Bolund, Lars and K?lvraa, Steen and Nielsen, Kirsten Vang and Nex?, Bj?rn A

Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2005): 80-6

[Comparative genomic hybridization analysis of nasopharyngeal carcinoma drug-resistant cell CNE2/DDP and its parent cell CNE2].

Authors: Jiang, Run-De and Hu, Liang and Guan, Xin-Yuan and Zhang, Li-Xin and Yue, Wen and Cen, Xin-Tang and Li, Chun-Hai

Journal: Ai zheng = Aizheng = Chinese journal of cancer (2004): 386-90

Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.

Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong

Journal: Biotechnology and bioengineering (2003): 127-33