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超氧化物歧化酶SOD活性分析試劑盒

發布者:艾美捷科技    發布時間:2021-06-25     
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超氧化物歧化酶.jpg

 

超氧化物歧化酶(Superoxide dismutase,SOD)是一種廣泛存在于動物、植物和微生物中的抗氧化的金屬酶,按照結合金屬離子種類不同,該酶有以下三種:含銅與鋅超氧化物歧化酶(Cu/Zn-SOD)、含錳超氧化物歧化酶(Mn-SOD)和含鐵超氧化物歧化酶(Fe-SOD),三種 SOD 都催化超氧化物陰離子自由基歧化生成過氧化氫與氧氣。

 

由于細胞自由基非?;顫姡瑓⑴c一系列的化學反應,能引起細胞生物膜上的脂質過氧化,破壞了膜的結構和功能,使體內的許多酶及激素失去生物活性,同時還能破壞核酸結構和導致整個機體代謝失常與病變。

 

作為專業的生命科學醫藥原料供應商,Cell Biolabs中國區金牌代理,艾美捷科技為您推薦:超氧化物歧化酶SOD活性分析試劑盒

 

超氧化物歧化酶SOD活性分析試劑盒.jpg

產品超氧化物歧化酶SOD活性分析試劑盒
OxiSelec Superoxide Dismutase Activity Assay
貨號STA-340 (100 assays)
檢測方法比色法
樣本類型懸浮細胞,貼壁細胞,組織裂解液,血清/血漿
檢測范圍1.2 mU/ul — 5U/ul
說明書下載點擊下載
實驗原理通過使用黃嘌呤(Xanthine)/黃嘌呤氧化酶(XOD)體系生成超氧化物陰離子。加入發色基團,發色基團可被由上述體系產生的氧化物陰離子還原成為水溶性的黃色甲染料,這樣SOD活性通過抑制發生基團的還原來測定。當存在SOD時,超氧陰離子濃度降低,產生的顏色信號下降。
試劑盒組分1. SOD Standard
2. Xanthine Solution
3. Xanthine Oxidase Solution, 150X
4. Chromagen Solution
5. SOD Assay Buffer, 10X
保存條件收到后,將試劑盒保存在-20℃,避免多次反復凍融

* 本產品僅適用于科研用途.

 

FAQ常見問題:

1.超氧化物歧化酶SOD活性分析試劑盒,有物種種屬限制嗎?

我們的SOD活性試劑盒,適用于任何生物蛋白質樣品,因為它使用黃嘌呤/黃嘌呤 氧化酶方法測量酶活性,并不依賴于可能因物種而異的蛋白質結構。

 

2.是否可以檢測凍存過的樣本?

我們的超氧化物歧化酶SOD活性分析試劑盒通常適用于在 -80?C 下保存兩個月以內樣品,但我們建議盡可能測試新鮮樣品以獲得最佳結果。

 

3.你們的超氧化物歧化酶SOD活性分析試劑盒可以檢測3種SOD酶嗎?

我們的SOD活性試劑盒,可以檢測所有類型的SOD酶,包括含銅與鋅超氧化物歧化酶Cu/ Zn-SOD,含錳超氧化物歧化酶Mn-SOD,以及含鐵超氧化物歧化酶Fe-SOD。

 

結果展示:

SOD試劑盒標準曲線.png

SOD試劑盒標準曲線

 

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