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特色產品TG2重組蛋白/Recombinant TG2英文說明書:
Art. No.:T122
Synonym:Tissue-type Transglutaminase, TG2, TGase 2, protein-glutamine-γ-glutamyltransferase, His6-rhTG2
Molecular Weight:78 kDa
Source:Recombinantly produced in insect cells
Purity:> 95% [SDS-PAGE and Coomassie staining]
The gel shows a single dominant protein band at ~80 kDa.
Appearance:White lyophilized solid
Reagents:The Transglutaminase is lyophilized from 10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL Maltodextrin.
Reconstitution:Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. Keep cooled on ice for short term storage.
Application:The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.
Coating:Dilute with your coating buffer to an appropriate concentration e.g. 1 μg/ml. Please notice that coating conditions have to be evaluated carefully.
Storage:T122 is stable for at least two years if stored at ≤ -20°C.
Delivery is possible at ambient temperature
Note:Intended for research use only, not for use in human, therapeutic or diagnostic applications.
艾美捷TG2重組蛋白/Recombinant TG2中文說明書:
產品編號:T122
同義詞:組織型轉谷氨酰胺酶,TG2,TGase 2,蛋白谷氨酰胺γ-谷氨酰轉移酶,His6-rhTG2
分子量:78 kDa
來源:在昆蟲細胞中重組生產
純度:
95% [通過SDS-PAGE和Coomassie染色檢測]
凝膠顯示一個主要的蛋白條帶,大約在80 kDa。
外觀:白色凍干固體
試劑:轉谷氨酰胺酶是從10 mM Tris-HCl pH 8.1, 150 mM NaCl, 5 mM DTT, 1 mM EDTA, 189 mg/mL 麥芽糊精中凍干得到的。
復溶:向凍干粉末的小瓶中加入與蛋白質凍干前相同的體積的水(見分析證書)。輕輕旋轉小瓶直到固體溶解。復溶后,溶液應分裝后冷凍儲存。短期儲存時,請保持在冰上冷卻。
應用:重組抗原適用于固相(酶聯免疫吸附試驗ELISA和免疫印跡)和液相診斷檢測。該蛋白可被人IgA和IgG(自身)抗體結合。
包被:用您的包被緩沖液稀釋至適當濃度,例如1 μg/ml。請注意,包被條件需要仔細評估。
儲存:如果儲存在≤ -20°C,T122至少穩定兩年??梢栽诔叵陆桓丁?/p>
注意:僅供研究使用,不適用于人體、治療或診斷應用。

僅供研究使用,不適用于人體、治療或診斷應用。

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