PRODUCT CENTER
特色產品酶標記可以通過酶組織化學方法通過產生顏色的反應(色原反應)或產生熒光的反應(熒光反應)來可視化。最常用的酶是辣根過氧化物酶(HRP)。HRP可以通過色原反應(如二氨基聯苯胺,例如DAB)、熒光(例如ADHP)或化學發光(例如魯米諾)來可視化。高度純化和交叉吸收純化的IgG在中性條件下與HRP高效率偶聯,以最大限度地保留IgG和HRP的活性,使用AAT Bioquest專有的Buccutite?蛋白交聯技術。

圖:用泛角蛋白小鼠單克隆抗體標記石蠟包埋的人肺癌的免疫熒光圖像,隨后用HRP標記的山羊抗小鼠IgG(H+L)標記(Cat#16728)。該信號是用AAT的iFluor?488酪胺(目錄號11060,綠色)產生的。細胞也用DAPI(藍色)復染。
艾美捷AAT Bioquest-HRP標記的山羊抗小鼠IgG(重鏈+輕鏈):
單位大?。? 毫克
目錄編號:16728
分子量:~150000
溶劑:水
H-短語:H303, H313, H333
危害符號:XN
預期用途:僅限研究使用(RUO)
R-短語:R20, R21, R22
儲存:冷凍(< -15°C);盡量減少光照
UNSPSC:12171501
AAT Bioquest-HRP標記的山羊抗小鼠IgG(重鏈+輕鏈)文獻參考:
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
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Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
Authors: Boonrod K, Roth B, Leong Ngar S, Krczal G.
Journal: Anal Biochem (2010): 124
艾美捷科技是AAT Bioquest的中國代理商,為科研工作者提供優質的產品與服務。

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