SUMOylation substrate. Recombinant protein expressed with N-terminal His-tag.
IRF2 interacts with the SUMO-E3 ligase PIASy and is SUMOylated in vivo. This SUMO modification regulates the transcriptional activities of IRF2 with mutagenetic analysis suggesting that it contains three SUMOylation sites. SUMOylation of IRF2 has no significant effects on its nuclear localization or DNA binding activity, but increases its ability to inhibit IRF1 transcriptional activity and decreases its ability to activate the ISRE and histone H4 promoters.
