Ubc12, also known as UBE2M, is the major NEDD8 E2 conjugating enzyme and an essential component of the NEDD8 cascade.
Ubc12 is responsible for conjugating activated NEDD8 and mediating its subsequent linkage, via C-terminal isopeptide bond formation, to a number of proteins, including the tumour suppressors p53 and VHL, in addition to its well characterised modification of members of the cullin ubiquitin E3 ligase family. Such modifications allow NEDD8 to play a critical regulatory role in cell proliferation and development, a process closely associated to the ubiquitin-proteasome pathway.
Recombinant human Ubc12 protein expressed with a N-terminal His-tag. Protein has utility in NEDDylation reactions and polyNEDD8 chain synthesis.
