- 中文名稱
Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit
- 英文名字
- Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- T5585-75
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- 產品描述
- The TIMP-2 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same TIMP-2 molecule. Upon addition of a TIMP-2-containing specimen, the result is a TIMP-2 molecule being sandwiched between the solid phase and enzyme labeled antibodies. After removing unbound enzyme-labeled antibody, the bead containing the sandwich is incubated with enzyme substrate and TMBZ, resulting in the development of color. The activity of peroxidase enzyme is proportional to the amount of antigen, TIMP-2, so that TIMP-2 concentration in specimens can be determined from a standard curve. The TIMP-2 immunoassay kit is useful for the determination of TIMP-2 levels in fresh serum and conditioned medium of cells. This system does not recognize TIMP-2 complexed with proMMP-2. While the immunoreactivities of TIMP-2 complexed with active MMP-1, MMP-2 or MMP-9 are increased by approximately 300%, 450%, and 300%, respectively, compared with that of the free form of TIMP-2, no change is observed when TIMP-2 is complexed with active MMP-3. Contents of this kit are sufficient for assay of 100 samples, including standard curve. Testing of samples in duplicate or triplicate is strongly recommended. This kit is intended for research use only; not for diagnostic or therapeutic applications. Sensitivity: 20ng/ml Range of Detection: 20ng/ml – 320ng/mlKit Components:1. Anti-TIMP-2 Coated Beads–One (1) bottle containing 100 anti-TIMP-2 coated polystyrene beads.2. Concentrated Enzyme Labeled Antibody Solution–One (1) 1ml bottle containing TIMP-2 antibody.3. Color Reagent– One (1) 1ml bottle 3,3',5,5'-Tetramethylbenzidine (TMBZ)4. Buffer for Color Reagent: 40ml Acetate buffer, pH 5.5.5. Substrate Solution–One (1) 15ml bottle 0.02% Hydrogen Peroxide.6. Stop Solution–Two (2) bottles, sufficient to make 100ml 1.3 N Sulfuric acid per bottle.7. Buffer Reagent (Lyophilized)–One (1) 50ml bottle BSA in sodium phosphate buffer.8. TIMP-2 Standard (Lyophilized)–One (1) bottle, sufficient to make 1ml Human TIMP-2 Standard.9. Concentrated Wash Solution–Two bottles, sufficient to make 1L Sodium phosphate buffer, pH 7.0, per bottle.Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產品特點
- 針對Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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