- 中文名稱
Thyroxine, Free, Human, BioAssay ELISA Kit (T4)
- 英文名字
- Thyroxine, Free, Human, BioAssay ELISA Kit (T4)
- 供應商
- USBiological
- 產品貨號
- T5460-10B
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- 產品描述
- The United States Biological Thyroxine, Free, Human, BioAssay ELISA Kit is for the quantitative determination of free thyroxine concentration in human serum.Background:Thyroxine, the principal thyroid hormone, circulates in blood almost completely bound to carrier proteins. The main carrier is thyroxine-binding globulin (TBG). However, only the free (unbound) portion of thyroxine is responsible for the biological action. Further, the concentrations of the carrier proteins are altered in many clinical conditions, such as pregnancy. In normal thyroid function as the concentrations of the carrier proteins alters, the total thyroxine level changes so that the free thyroxine concentration remains constant. The increase in total thyroxine associated with pregnancy, oral contraceptives and estrogen therapy occasionally result in total T4 levels over the limits of normal while the free thyroxine concentration remains in the normal reference range. Masking of abnormal thyroid function can also occur in both hyper and hypothyroid conditions by alterations in the TBG concentration. The total T4 can be elevated or lowered by TBG changes such that the normal reference levels result. In this method, serum reference, a control is first added to a microplate well. Enzyme-T4 conjugate (analog method) is added and the reactants are mixed. A competition reaction results between the enzyme conjugate and the free thyroxine for a limited number of antibody combining sites immobilized on the well. After the completion of the required incubation period, the antibody bound enzyme-thyroxine conjugate is separated from the unbound enzyme-thyroxine conjugate via a wash step. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color. The employment of several serum references of known free thyroxine concentration permits construction of a graph of activity and concentration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with free thyroxine concentration. Kit Components: 1. Serum References: 6x1ml 2. fT4 (HRP) Reagent: 1x13ml3. Microtiter Plate: 1x96 wells4. Wash Solution Concentrate: 1x20ml5. Tetramethylbenzidine (TMB) A: 1x7ml 6. Tetramethylbenzidine (TMB) B: 1x7ml 7. Stop Solution: 1x8ml (1N HCl) Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 產品特點
- 針對Thyroxine, Free, Human, BioAssay ELISA Kit (T4)該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Thyroxine, Free, Human, BioAssay ELISA Kit (T4)產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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