- 中文名稱
Thyroid Stimulating Hormone, Human, Normal (TSH) BioAssay ELISA Kit
- 英文名字
- Thyroid Stimulating Hormone, Human, Normal (TSH) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- T5400-01B
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- 產品描述
- Intended Use:This Human TSH ELISA Kit is to be used for the in vitro quantitative determination of human thyroid stimulating hormone (TSH) concentrations in serum. It is intended for research use only and not for use in diagnostic procedures.Principle of the Assay:This TSH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TSH. Standards or samples are then added to the microtiter plate wells and TSH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TSH are added to each well to “sandwich” the TSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TSH and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. In order to measure the concentration of TSH in the sample, this Human TSH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus TSH concentration (uIU/ml). The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: <0.2uIU/mlRange: 0.2-40uIU/mlKit Components:T5400-01B1: Microtiter Plate, 1x 96 wellsT5400-01B12: Conjugate, 1x 12mlT5400-01B13. Standard: 40uIU/ml, 1x1vialT5400-01B14. Standard: 20uIU/ml, 1x1vialT5400-01B15. Standard: 10uIU/ml, 1x1vialT5400-01B16. Standard: 5uIU/ml., 1x1vialT5400-01B17. Standard: 1uIU/ml, 1x1vialT5400-01B18. Standard: 0uIU/ml, 1x1vialT5400-01B19. Substrate A, 1x10mlT5400-01B110. Substrate B, 1x10mlT5400-01B111. Stop Solution, 1x7ml. 2N sulfuric acid. Materials required but not provided:1. Single or multi-channel precision pipettes with disposable tips: 10-10ul and 50-200ul for running the assay.2. Pipettes: 1ml, 5ml, and 10ml for reagent preparation. 3. Multi-channel pipette reservoir or equivalent reagent container. 4. Test tubes and racks. 5. Polypropylene tubes or containers (25ml). 6. Incubator (37±2°C) 7. Microtiter plate reader (450 nm±2 nm) 8. Automatic microtiter plate washer or squirt bottle 9. Sodium hypochlorite solution, 5.25% (household liquid bleach). 10. Deionized or distilled water 11. Plastic plate cover12. Disposable gloves13. Absorbent paperStorage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產品特點
- 針對Thyroid Stimulating Hormone, Human, Normal (TSH) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Thyroid Stimulating Hormone, Human, Normal (TSH) BioAssay ELISA Kit產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
-
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
-
