Thromboxane B2 (TXB2) is a stable hydrolyzed product of unstable TXA2 which is derived from PGH2. PGH2 is synthesized from arachidonic acid through the cyclooxygenase pathway. It is a major product following platelet aggregation induced by a variety of agents such as thrombin and collagen. It is produced not only in platelets, but also in other cell types such as fibroblasts and macrophages. Quantitation of thromboxane formation can be made by determining the level of TXB2.Kit components:T5120-02A EIA Buffer: 1x30ml. To be used to dilute enzyme conjugate and TXB2 standards.T5120-02B Wash Buffer (10X): 1x20ml. To be diluted 10x with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.T5120-02C Substrate: 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.T5120-02D Extraction Buffer (5X): 1x30ml. To be diluted 5x with deionized water. This is used for diluting extracted and non-extracted samples.T5120-02E Thromboxane B2 (HRP): 1x150ul. TXB2 HRP concentrate. Blue capped vial.T5120-02F Standard: 1x100ul. TXB2 standard at the concentration of 1ug/ml. Green capped vial.T5120-02G Microtiter Plate:1x96 wells Nunc microplate with anti-TXB2 rabbit antibody precoated on each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
