- 中文名稱
Thromboxane 11-H2 (11-Dehydrothromboxane; 11-d-TBX2) BioAssay ELISA Kit
- 英文名字
- Thromboxane 11-H2 (11-Dehydrothromboxane; 11-d-TBX2) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- T5113
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- 產品描述
- 11-Dehydro-Thromboxane B2 (11-D-TXB2) is derived from TXB2 by the action of 11-dehydrogenase. It is one of the major metabolites of TXB2 in plasma and urine. Measurement of 11-D-TXB2 in plasma represents an approach to the assessment of thromboxane formation in the human circulation.Principle of the Assay:This is an ELISA for the quantitative analysis of 11-DT-hromboxane B2 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the 11-D-TXB2 in the sample for a limited number of binding sites on the antibody coated plate. The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of 11-D-TXB2 in the sample or standard. For example, the absence of 11-D-TXB2 in the sample will result in a bright blue color, whereas the presence of 11-DTXB2 will result in decreased or no color development.Kit Components:1. EIA Buffer, 1x30ml. To be used to dilute enzyme conjugate and 11-D-TXB2 standards.2. Wash Buffer (10X), 1x20ml. To be diluted 10X with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.3. Substrate, 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.4. Extraction Buffer (5X), 1x30ml. To be diluted 5X with deionized water. This is used for diluting extracted and non-extracted samples.5. 11-Dehydro-Thromboxane B2 Enzyme Conjugate, 1x150ul. 11-D-TXB2 HRP concentrate. Blue capped vial.6. 11-Dehydro-Thromboxane B2 Standard, 1x100ul. 11-D-TXB2 standard at the concentration of 1ug/ml.7. 11-Dehydro-Thromboxane B2 Antibody Coated Plate, 1x 96 well microplate with anti-11-D-TXB2 rabbit antibody precoated on each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產品特點
- 針對Thromboxane 11-H2 (11-Dehydrothromboxane; 11-d-TBX2) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Thromboxane 11-H2 (11-Dehydrothromboxane; 11-d-TBX2) BioAssay ELISA Kit產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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