Oxidative Stress/ Damage->DNA / RNA Damage and Repair->UV Induced DNA Damage Kits
應用類型
Our OxiSelect? Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
免疫原
Measure CPD structures of DNA lesions induced by UV light
來源宿主
OxiSelect? 細胞紫外線誘導 DNA 損傷染色套件(CPD)(OxiSelect? Cellular UV-Induced DNA Damage Staining Kit (CPD))保存建議,請查看該產品的使用手冊