SPY505-CA is the ChloroAlkane (CA) derivative of our SPY505 fluorophore. It emits light in the green part of the UV-ViS spectrum, it is fluorogenic, highly cell permeable and well suited for STED and SIM superresolution imaging. SPY505-CA can be imaged with a standard FITC or YFP filterset. It can be used for widefield, confocal, SIM or STED imaging in living or fixed cells and tissue. It has the same structure as MaP510-halo, the name used in its original publication in Nature Chemistry. ChloroAlkane (CA) is the substrate of the self labeling tag Halo-tag?*. Upon reaction with a CA derivative, Halo-tag?* forms a covalent bond with the substrate. It allows to permanently attach a fluorescent label to any protein of interest (POI) expressed as Halo-tag?* fusion. Contains 1 vial of SPY505-CA (lyophilized). *Halo-tag? is a registered trademark of Promega Corporation.
