SIRT1, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-1, hSIRT1, Regulatory Protein SIR2 Homolog 1, SIR2-like Protein 1, hSIR2, SIR2L1)-SIRT1, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-1, hSIRT1, Regulatory Protein SIR2 Homolog 1, SIR2-like Protein 1, hSIR2, SIR2L1)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: SIRT1, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-1, hSIRT1, Regulatory Protein SIR2 Homolog 1, SIR2-like Protein 1, hSIR2, SIR2L1)

英文名字: SIRT1, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-1, hSIRT1, Regulatory Protein SIR2 Homolog 1, SIR2-like Protein 1, hSIR2, SIR2L1)

供應商: USBiological

產品貨號: S1113-81

產品報價: ￥1/100Tests

產品說明書: 點擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！

產品描述: Sirtuin 1 is the human ortholog for the yeast Sir2 (silent information regulator 2) protein regulating epigenetic gene silencing as a possible antiaging effect. Sirtuin 1 is an NAD(+)-dependent histone deacetylase, which deacetylate lysines 9 and 14 of histone H3 and lysine-16 of histone H4, involved in various cellular functions such as transcription, energy sensing, and differentiation. Sirtuin1 plays an important role in regulating adipogenesis via repression of PPAR and the gluconeogenic/glycolytic pathways in liver in response to fasting signals through the transcriptional coactivator PGC1A deacetylated at specific lysine residues in an NAD(+)-dependent manner. This assay is a sandwich ELISA for quantitative determination of human Sirtuin 1 in cells. A monoclonal antibody specific for Sirtuin 1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Sirtuin 1 is recognized by the addition of a purified polyclonal antibody specific for Sirtuin 1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of Sirtuin 1 in the samples. This ELISA is specific for the measurement of natural and recombinant human Sirtuin 1. It does not cross-react with human Sirtuin 2, human Sirtuin 5, human Sirtuin 6, human adiponectin, human resistin, human RBP4, human vaspin, human progranulin, human GPX3, human FTO, human Nampt, human leptin, mouse FTO, mouse Nampt. The assay range is 0.031-2ng Sirtuin 1/ml and a detection limit of 30pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).Sample Type:Cell lysates.Intended Use:For the quantitative determination of human Sirtuin 1 in cells.Sensitivity:30pg/ml Range:0.031-2ng Sirtuin 1/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of human Sirtuin 1 in cells. A monoclonal antibody specific for Sirtuin 1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Sirtuin 1 is recognized by the addition of a purified polyclonal antibody specific for Sirtuin 1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of Sirtuin 1 in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlLysis Buffer (10X): 1x12mlDetection Antibody: 1x12mlDetector 100X (HRP conjugated anti-IgG): 1x150ulHuman Sirtuin 1 Standard (lyophilized): 4ngHuman Sirtuin 1 QC Sample (lyophilized): 1 vialSubstrate Solution (TMB): 1x12mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對SIRT1, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-1, hSIRT1, Regulatory Protein SIR2 Homolog 1, SIR2-like Protein 1, hSIR2, SIR2L1)該產品的特點優勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到SIRT1, Human, BioAssay ELISA Kit (NAD-dependent Protein Deacetylase Sirtuin-1, hSIRT1, Regulatory Protein SIR2 Homolog 1, SIR2-like Protein 1, hSIR2, SIR2L1)產品后將其置于4°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。