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中文名稱

S6 Ribosomal Protein, phosphorylated (Ser235/236) BioAssay ELISA Kit discontinued

英文名字
S6 Ribosomal Protein, phosphorylated (Ser235/236) BioAssay ELISA Kit discontinued
供應商
USBiological
產品貨號
R2031-29F
產品報價
¥1/1Kit
產品說明書
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購買方式
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業??梢詾槟峁┘皶r的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
To effectively promote growth and cell division in a sustained manner, growth factors and mitogens must upregulate translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase, which in turn phosphorylates the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation, particularly of mRNAs with an oligopyrimidine tract in their 5' untranslated regions (2). This group of mRNAs (5'TOP) encodes proteins involved in cell cycle progression and proteins that are part of the translational machinery, such as ribosomal proteins and elongation factors (2,3). The main in vivo S6 ribosomal protein phosphorylation sites, including Ser235, Ser236, Ser240 and Ser244, are located within a small 19 amino acid region in the S6 carboxy terminus (4,5).Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Antibody Pair is being offered as an alternative to our Phospho-S6 Ribosomal Protein (Ser235/236) Sandwich ELISA Kit. Capture and Detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are provided for performing 4 x 96 well ELISAs. The S6 Ribosomal Protein Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added, followed by PhosphoS6 Ribosomal Protein (Ser 235/236) Detection Antibody and HRP-conjugated secondary antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance at 450 nm is proportional to the quantity of phospho-S6 Ribosomal protein (Ser235/236) protein. *Antibodies in this kit are custom formulations specific to the kit.Kit Components:S6 Ribosomal Protein Capture Antibody (100X) 1x0.4ml Phospho-S6 Ribosomal Protein (Ser235/236) Detection Antibody (100X) 1x.4ml Anti-Rabbit IgG HRP-Linked Antibody (1000X), 1x0.04ml Storage and Stability:S6 Ribosomal Protein Capture Antibody and Phospho-S6 Ribosomal Protein (Ser 235/236) Detection Antibody are stored at 4°C. Anti-Rabbit IgG-HRP Linked Antibody is stored at -20°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.? Reagents not supplied: Phosphate Buffered Saline (PBS-20X)Phosphate Buffered Saline with Tween -20 (PBST-20X)Cell Lysis Buffer (10X)TMB SubstrateSTOP SolutionBlocking Buffer- PBS+0.05% Tween-20, 1% BSA 96 Well MicroplatesMicroplate Reader
產品特點
針對S6 Ribosomal Protein, phosphorylated (Ser235/236) BioAssay ELISA Kit discontinued該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到S6 Ribosomal Protein, phosphorylated (Ser235/236) BioAssay ELISA Kit discontinued產品后將其置于-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
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