Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)-Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)

英文名字: Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)

供應商: USBiological

產品貨號: R1585-01W

產品報價: ￥1/100Tests

產品說明書: 點擊查看

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背景資料: USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！

產品描述: Obesity is a well-known risk factor of type 2 diabetes mellitus and is strongly associated with insulin resistance. Resistin (also called FIZZ3/ADSF) is an adipocyte-derived peptide first identified during a search for targets of thiazolidinediones. Serum concentrations of resistin are markedly increased in obese mice and are decreased by treatment with thiazolidinediones. It was also found that administration of an anti-resistin antibody increases insulin-stimulated glucose uptake in obese mice and that treatment of normal mice with recombinant resistin impairs insulin action. Thus, resistin might link obesity with insulin resistance and diabetes in mice models. However, subsequent studies in rodent models have produced disparate findings on the role of resistin in obesity and insulin resistance. In humans, while the expression of resistin in human adipocytes is very low compared with that seen in rodents and does not differ between normal, insulin-resistant or type 2 diabetic individuals, a more recent study using a large size of case suggests that the plasma resistin levels are increased in type 2 diabetes. Therefore determination of the plasma resistin levels may be important for understanding onsets of metabolic diseases such as type 2 diabetes or obesity. This assay is a sandwich ELISA for quantitative determination of mouse resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of resistin in the samples. The assay range is 0.47-30ng/ml resistin/ml. The lowest level of resistin that can be detected by this assay is 100pg/ml.Sample Type:Cell and tissue culture supernatants, urine, plasma, serum, as well as many other biological fluids.Intended Use:For the quantitative determination of mouse Resistin in biological fluids.Sensitivity:100pg/mlRange:0.47-30ng/ml Resistin/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of mouse resistin in biological fluids. A monoclonal antibody specific for resistin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, resistin is recognized by the addition of a biotinylated polyclonal antibody specific for resistin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of resistin in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlDetection Antibody: 1x12mlDetector 100X (HRP Labeled Streptavidin): 1x150ulMouse Resistin Standard (lyophilized): 16ngMouse Resistin QC Sample (lyophilized): 1 vialSubstrate Solution I (TMB): 1x6mlSubstrate Solution II (Peroxidase): 1x6mlStop Solution: 1x12mlStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)該產品的特點優勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到Resistin, Mouse, BioAssay ELISA Kit (Adipose Tissue-specific Secretory Factor, ADSF, Adipose-specific Cysteine-rich Secreted Protein A12-alpha, Cysteine-rich Secreted Protein FIZZ3, Fizz3, Retn)產品后將其置于4°C保存。

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