DFF is a heterodimeric protein of 40kDa （DFFB） and 45kDa （DFFA） subunits. DFFA （DNA fragmentation factor subunit alpha） is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF is activated once DFFA is cleaved by caspase-3. The cleaved fragments of DFFA detach from DFFB （the active component of DFF）， which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units.
A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.
DFFA at chromosome 1 shows rare allelic variants in neuroblastoma tumors.
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大腸桿菌中重組表達
溶解建議
Sterile Filtered colorless solution. The DFFA solution contains 20mM Tris-HCl buffer （pH 8.0）， 10% glycerol and 1mM DTT.
保存建議
DFFA although stable 4℃ for 4 weeks， should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
