HMGB1 is an abundant chromatin-binding protein found in eukaryotic cell nucleus and acts in the assembly of nucleoprotein complexes. Inside the cell， HMGB1 binds to DNA and is involved in transcriptional regulation. Outside the cell， HMGB1 acts as a cytokine with activities that resemble those of tumor necrosis factor （TNF）. HMGB1 is elevated significantly in chronic kidney disease patients and correlates with glomerular filtration rate as well as with markers of inflammation and malnutrition. HMGB1 is involved in Gram-negative sepsis by catalyzing movement of LPS monomers from LPS aggregates to CD14 to initiate a TLR4-mediated proinflammatory response. HMGB1 plays an important role in the relationship between necrosis and malignancy in glioma tumours. HMG1 protein is induced by Mycobacterium bovis BCG. Overexpression of HMGB1 is common in gastrointestinal stromal tumors and is related to the KIT mutation. HMG1 induces growth inhibition and apoptosis in macrophages through RAGE intracellular signaling pathway. The increase of extracellular HMGB1 observed in salivary glands of Sjogren’s syndrome patients indicates that HMGB-1 is involved in the inflammatory process of the disease. HMGB-1 together with estrogen increase cell cycle progression in tumor cell lines.
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來源宿主
昆蟲Hi-5細胞中重組表達
溶解建議
Sterile Filtered colorless solution. The HMG1 solution （1mg/ml） contains 20mM Tris pH-8， 1mM EDTA， 0.5mM DTT and 10% glycerol.
保存建議
HMG1 although stable 4℃ for 4 weeks， should be stored desiccated below -18℃.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
