Interleukin 2 is a protein that has a variety of immunologic functions, the most notable being the ability of IL-2 to promote the proliferation and maturation of activated T cells. A comparison of the amino acid sequences of human and murine IL-2 shows an approximately 60% sequence similarity, and human sequence IL-2 has been found to be active on murine cell lines. Some of the biological activities attributed to IL-2 include:
● induction of secretion of interferon-γ and tumor necrosis factors -α and -β from peripheral blood mononuclear cells
● stimulation of the rate of synthesis of c-myc RNA and transferrin receptor
● activation of neutrophils
● stimulation of proliferation and maturation of activated helper T cells
● stimulation of proliferation of activated and natural killer cells and tumor-infiltrating lymphocytes, as well as enhancement of the ability of these cytotoxic lymphocytes to kill target cells
● induction of the expression of IL-2 receptors on T cells
● stimulation of proliferation of antibody-producing B cells
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20?C. Further dilutions should be made in appropriate buffered solutions.
保存建議
This lyophilized preparation is stable at 2-8?C, but should be kept at -20?C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8?C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20?C to -70?C. Avoid repeated freeze/thaw cycles.
