- 中文名稱
Protein Kinase C Assay Kit, BioAssay (PKC) discontinued
- 英文名字
- Protein Kinase C Assay Kit, BioAssay (PKC) discontinued
- 供應商
- USBiological
- 產品貨號
- P9105-98
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The assay kit is designed to measure the phosphotransferase activity of protein kinase C (PKC) in immunoprecipitates and column fractions. It is highly specific for alpha, beta and gamma. Crude cell lysates may also be used but detergents/biochemicals contained in the cell lysis buffer may inhibit PKC kinase activity. Furthermore, although two inhibitors are included with the kit, editors may suggest other unknown kinases found in crude lysates are responsible for substrate peptide phosphorylation. The assay kit is based on phosphorylation of a specific substrate peptide (QKRPSQRSKYL) using the transfer of the -phosphate of adenosine-5'-[32P] triphosphate ([ -32P] ATP) by PKC kinase. The phosphorylated substrate is then separated from the residual [ -32P] ATP using P81 phosphocellulose paper and quantitated by using a scintillation counter. The assay is linear for incubation times of up to 30 minutes and incorporation of up to 20% of total ATP. Further incubation or incorporation may not be linear and may therefore not be a true indication of PKC activity in the sample extract. The enzyme assay is rapid, convenient and fairly specific for PKC. Capacity:~200 PKC kinase assays. Kit Components: 1. Assay Dilution Buffer II (ASBII) containing 20mM MOPS, pH 7.2, 25mM β-glycerol phosphate, 1mM sodium orthovanadate, 1mM dithiothreitol, 1mM CaCl , 2x1ml2. PKC Substrate Cocktail, 500uM PKC substrate peptide [QKRPSQRSKYL] in ADBII, 2x1ml3. PKA/CaMK Inhibitor Cocktail containing 2uM PKA inhibitor peptide, and 20uM Compound R24571 in ADBII, 2x1ml4. PKC Lipid Activator containing 0.5mg/ml phosphatidyl serine and 0.05mg/ml diacylglycerol in ADBII, 2x1ml5. Mg/ATP Cocktail containing 75mM magnesium chloride and 500um ATP 20mM MOPS, pH 7.2, 25mM β-glycerophosphate, 5mM EGT A, 1mM Na3VO4, 1mM dithiothreitol, 2x1ml. (Before starting the assay, 90ul of the Mg2+/ATP cocktail should be added to 100uCi (10ul) of the [γ-32P]ATP (～3000Ci/mmol).)6. p81 squares, 1x200 eachStorage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?Other components required but not included as part of kit are: Sample preparation containing Protein Kinase C: 10-200ug protein/immunoprecipitate diluted into assay dilution buffer or 25-100ng of purified Protein Kinase C vortex mixer plexiglass shielding 30°C incubating water bath timer variable volume (5-200ul) pipet + tips 0.75% phosphoric acid scintillation vials scintillation fluid scintillation counter [gamma-32P]ATP-3000 Ci/mmol,
- 產品特點
- 針對Protein Kinase C Assay Kit, BioAssay (PKC) discontinued該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Protein Kinase C Assay Kit, BioAssay (PKC) discontinued產品后將其置于-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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