- 中文名稱
Prostaglandin F2 alpha 13,14-H2-15-K (PGF2a) BioAssay ELISA Kit
- 英文名字
- Prostaglandin F2 alpha 13,14-H2-15-K (PGF2a) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- P9053-34C
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- 13,14-dihydro-15-keto-Prostaglandin F2a (13,14-d-15-k-PGF2a) is a major metabolite of PGF2a in tissues. It is derived from PGF2a through the consecutive actions of 15-hydroxy prostaglandin dehydrogenase and prostaglandin Delta13-reductase. Because of the rapid metabolism of PGF2a, quantitation of 13, 14-d-15-k-PGF2a in circulation can be a good index for PGF2a synthesis in tissue.Principle of the Assay:This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of13,14-dihydro-15-keto-Prostaglandin F2a levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the 13,14-d-15-k-PGF2a in the sample for a limited number of binding sites on the antibody coated plate. The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of 13,14-d-15-k-PGF 2a in the sample or standard. For example, the absence of 13,14-d-15-k-PGF 2a in the sample will result in a bright blue color, whereas the presence of 13,14-d-15-k-PGF2a will result in decreased or no color development.Materials Provided:1. EIA Buffer, 1x30ml. To be used to dilute enzyme conjugate and 13,14-d-15-k-PGF2a standards.2. Wash Buffer (10X), 1x20ml. To be diluted 10x with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.3. Substrate, 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.4. Extraction Buffer (5X), 1x30ml. To be diluted 5x with deionized water. This is used for diluting extracted and non-extracted samples.5. 13,14 Dihydro-15-Keto-Prostaglandin F2 Enzyme Conjugate, 1x150ul. 13,14-d-15-k-PGF 2a HRP concentrate. Blue capped vial.6. 13,14 Dihydro-15-Keto-Prostaglandin F2 Standard, 1x100ul. 13,14-d-15-k-PGF 2a standard at the concentration of 1ug/ml. Green capped vial.7. 13,14 Dihydro-15-Keto-Prostaglandin F2 Antibody Coated Plate, 1x 96-well microplate with anti-13,14-d-15-k-PGF 2a rabbit antibody precoated on each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產品特點
- 針對Prostaglandin F2 alpha 13,14-H2-15-K (PGF2a) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Prostaglandin F2 alpha 13,14-H2-15-K (PGF2a) BioAssay ELISA Kit產品后將其置于-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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