- 中文名稱
Prostaglandin E2 (PGE2) BioAssay ELISA Kit
- 英文名字
- Prostaglandin E2 (PGE2) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- P9053-30
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Prostaglandin E2 (PGE2) is derived from PGH2 which in turn is synthesized from arachidonic acid through the cyclooxygenase pathway. Many cell types, such as epithelial cells, fibroblasts, and macrophages produce PGE2. PGE2 possesses vasoactivity, modulates immune funtions, regulates sleep-awake cycles and exhibits many other activities.Principle Of The Assay:This is an monoclonal ELISA (Enzyme-Linked ImmunoSorbent Assay) for the quantitative analysis of Prostaglandin E2 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the PGE2 in the sample for a limited number of binding sites on the antibody coated plate. The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of PGE2 in the sample or standard. For example, the absence of PGE2 in the sample will result in a bright blue color, whereas the presence of PGE2 will result in decreased or no color development.Kit Components:P9053-30A: EIA Buffer: 30ml. Provided to dilute enzyme conjugate and PGE2 standards.P9053-30B: Wash Buffer (10X): 20ml. Dilute 10 fold with deionized water. Diluted wash buffer is used to wash all unbound enzyme conjugate, samples and standards from the wells after the one hour incubation.P9053-30C: Substrate: 20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus Hydrogen Peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed. Light sensitive. Keep substrate refrigerated.P9053-30D: Extraction Buffer (5X): 30ml. Dilute 5 fold with deionized water. This buffer is used for diluting extracted and non-extracted samples.P9053-30E: Prostaglandin E2 Enzyme Conjugate: Two vials of lyophilized PGE2 HRP conjugate. Reconstitution with 75ul of deionized water results in a 50:1 concentrate. Blue capped vials.P9053-30F: Prostaglandin E2 Standard: 100ul. PGE2 standard provided at the concentration of 1ug/ml. Glass vial. Solution is viscous, take precaution when pipetting.P9053-30G: Prostaglandin E2 Antibody Coated Plate: A 96 well Costar microplate precoated with monoclonal mouse antibody against PGE2 The plate is ready for use as is. Do not wash.Storage and Stability:Store all components at 4°C except P9053-30E. Store lyophilized P9053-30E at -20°C. Store reconstituted P9053-30E at 4°C.
- 產品特點
- 針對Prostaglandin E2 (PGE2) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Prostaglandin E2 (PGE2) BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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