- 中文名稱
Prolactin, Human (LTH, Luteotropic Hormone, Lactogenic Hormone) BioAssay ELISA Kit
- 英文名字
- Prolactin, Human (LTH, Luteotropic Hormone, Lactogenic Hormone) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- P9009-02
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
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- 產品描述
- Human Prolactin (lactogenic hormone) is secreted from the anterior pituitary gland in both men and women. Human Prolactin is a single chain polypeptide hormone with a molecular weight of approximately 23,000. The release and synthesis of Prolactin is under neuroendocrinal control, primarily through Prolactin Releasing Hormone and Prolactin Inhibiting Hormone. Women normally have slightly higher basal Prolactin levels than men. Apparently, there is an estrogen-related rise at puberty and a corresponding decrease at menopause. The primary functions of Prolactin are to initiate breast development and to maintain lactation. Prolactin also suppresses gonadal function. During pregnancy, Prolactin levels increase progressively to between 10 and 20 times normal values, declining to non-pregnant levels by 3-4 weeks post-partum. Breastfeeding mothers maintain high levels of Prolactin, and it may take several months for serum concentrations to return to non-pregnant levels. The determination of Prolactin concentration is helpful in diagnosing hypothalamic-pituitary disorders. Microadenomas (small pituitary tumors) may cause hyperprolactinemia, which is sometimes associated with male impotence. High Prolactin levels are commonly associated with galactorrhea and amenorrhea. Prolactin concentrations have been shown to be increased by estrogens, thyrotropin-releasing hormone (TRH), and several drugs affecting dopaminergic mechanisms. Prolactin levels are elevated in renal disease and hypothyroidism, and in some situations of stress, exercise and hypoglycemia. Additionally, the release of Prolactin is episodic and demonstrates diurnal variation. Mildly elevated Prolactin concentrations should be evaluated taking these considerations into account. Prolactin concentrations may also be increased by drugs such as chloropromazine and reserpine and may be lowered by bromocriptine and L-dopa.This Prolactin enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for Prolactin. Standards or samples are then added to the microtiter plate wells and Prolactin if present, will bind to the antibody pre-coated on the wells. In order to quantitate the amount of Prolactin present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for Prolactin are added to each well to “sandwich” the Prolactin immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Prolactin and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. In order to measure the concentration of Prolactin in the sample, this Human Prolactin ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus Prolactin 3 concentration (ng/ml). The concentration of Prolactin in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: <1.5ng/mlRange: 1.5-200ng/mlKit Components:P9009-01A: Microtiter Plate1x96wellsP9009-01B: Prolactin (HRP)1x12mlP9009-01C: Standard-200ng/ml1x1vialP9009-01D: Standard -100ng/ml1x1vialP9009-01E: Standard-50ng/ml1x1vialP9009-01F: Standard-25ng/ml1x1vialP9009-01G: Standard-5ng/ml1x1vialP9009-01H: Standard-0ng/ml1x1vialP9009-01J: Substrate A1x10mlP9009-01K: Substrate B1x10mlP9009-01L: Stop Solution1x7mlStorage and Stability:Stored at 4°C. Stable for at least 6months.
- 產品特點
- 針對Prolactin, Human (LTH, Luteotropic Hormone, Lactogenic Hormone) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Prolactin, Human (LTH, Luteotropic Hormone, Lactogenic Hormone) BioAssay ELISA Kit產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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