尿液外泌體和自由循環RNA提取試劑盒（中提）-Urine Exosome and Free-Circulating RNA Isolation Midi Kit代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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產品描述: This kit provides a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different urine sample volumes ranging from 2 mL to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA.The kit provides a clear advantage over other available kits since they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

產品特點: Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA; Versatile sample input ranging from 2 mL to 10 mL; Isolate all sizes of free-circulating RNA, including microRNA?; Bind and elute all RNA irrespective of size or GC content, without bias; The purified exosomal RNA is free from any circulating RNA-binding proteins; No phenol extractions, Proteinase K treatment nor carrier RNA required; No time-consuming ultracentrifugation, filtration nor special syringes are required; No precipitation reagents nor overnight incubation required; Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 μL to 100 μL; Purify high-quality RNA in 15-20 minutes.

其他: Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術公司，基于專利的碳化硅膜純化技術和無苯酚專利配方，生產高品質的核酸和蛋白質純化及富集產品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發的科技創新獎，是一家致力于樣品制備并獲得ISO 9001（產品質量）、ISO 13485（產品可用于商業化的醫療設備，包括用于體外診斷領域）和ISO 15189（可用于臨床檢測機分子診斷領域的研發能力）認證的生物科技公司。目前該公司的產品多達700余種，提供一流的樣品制備試劑盒，應用于基因組和蛋白組等不同的領域。其產品主要覆蓋包括各種標本類型的DNA，RNA和蛋白質的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。