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細胞分析試劑盒
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中文名稱

血漿/血清循環核酸純化大提試劑盒(提取試劑盒)

英文名字
Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit (Slurry Format)
供應商
Norgen Biotek
產品貨號
NGB-53400
產品報價
¥已停產
產品說明書
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購買方式
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產品描述
Norgen's Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides a fast, reliable and simple procedure for isolating circulating nucleic acids (mRNA, miRNA, exosomal RNA, viral RNA, DNA and viral DNA) from various amounts of plasma/serum ranging from 2 mL to 5 mL.This kit is able to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA and circulating DNA. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating NA from large sample volumes. Free-Circulating NA can be isolated from either fresh or frozen samples using this kit, and the kit allows for the concentration of NA that is present in low concentrations (1-100ng/mL in human plasma). Typical yields of free-circulating NA will vary depending on the input sample, as the amount of NA present in plasma and serum will depend upon the health status of the individual and the level of nucleases present in the blood. This kit is suitable for the purification of Nucleic Acid from serum or plasma prepared from blood collected on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.Free-circulating nucleic acids in plasma and serum have the potential to provide biomarkers for certain cancers and disease states, and include tumor-specific extracellular nucleic acid fragments and fetal DNA in maternal blood. Free-circulating DNA is usually present as short fragments of less than 1000 bp, whereas free-circulating RNA and exosomal RNA in plasma and serum are usually present as short fragments of less than 1000 nt. In addition, plasma or serum also contains free-circulating miRNA that are as small as 21 nt. Furthermore, circulating viral DNA or viral RNA may be present in the plasma or the serum of viral-infected patients. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides an efficient method for the purification of all sizes of these fragmented free-circulating nucleic acids from human plasma or serum.
產品特點
Isolate all sizes of circulating and exosomal RNA, including microRNA; Isolate all sizes of circulating DNA from plasma and serum samples; Versatile plasma and serum input volumes (2 mL to 5 mL); Concentrate circulating nucleic acids into small elution volumes; Isolate inhibitor-free circulating nucleic acids; Bind and elute all RNA irrespective of size or GC content, without bias.
保存建議
所有緩沖液應密封保存,并在室溫(15-25°C)下保存長達1年。具體保存情況收到產品后請按說明書存放。
其他
Norgen Biotek成立于1998年,公司總部位于加拿大,是一家著名全球化生物技術公司,基于專利的碳化硅膜純化技術和無苯酚專利配方,生產高品質的核酸和蛋白質純化及富集產品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發的科技創新獎,是一家致力于樣品制備并獲得ISO 9001(產品質量)、ISO 13485(產品可用于商業化的醫療設備,包括用于體外診斷領域)和ISO 15189(可用于臨床檢測機分子診斷領域的研發能力)認證的生物科技公司。 目前該公司的產品多達700余種,提供一流的樣品制備試劑盒,應用于基因組和蛋白組等不同的領域。其產品主要覆蓋包括各種標本類型的DNA,RNA和蛋白質的純化富集,從活檢樣本(包括尿液,血漿,血清和血液)中純化RNA和DNA,全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。
注意
該頁面的中文產品信息的翻譯,僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前,請瀏覽說明書確認。
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