NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase -NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, QR1, DIA4, NMOR1)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase

英文名字: NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, QR1, DIA4, NMOR1)

供應商: USBiological

產品貨號: N5379-52

產品報價: ￥1/100Tests

產品說明書: 點擊查看

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產品描述: NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), a multifunctional antioxidant enzyme and exceptionally versatile cytoprotector, both protects the cell from carcinogenic oxidative damage and stabilizes the tumor suppressor p53 protein. At high glycolysis levels NQO1 stabilizes and protects p53 from ubiquitin-independent degradation, a process that is NADH dependent, and also elevates NAD+/NADH levels. NAD+ and NADH play a crucial role in cellular energy metabolism, and a dysregulated NAD+/NADH ratio is implicated in metabolic syndrome. In humans, NQO1 is expressed at high levels in adipocytes and its expression levels are positively correlated with adiposity, glucose tolerance, and liver dysfunction. Thus NQO1 may provide the basis for a new therapy for the treatment of metabolic syndrome. This assay is a sandwich ELISA for quantitative determination of human NQO1 in cells. A monoclonal antibody specific for NQO1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, NQO1 is recognized by the addition of a purified polyclonal antibody specific for NQO1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of NQO1 in the samples. This ELISA is specific for the measurement of natural and recombinant human NQO1. It does not cross-react with human adiponectin, human RBP4, human Nampt, human vaspin, human progranulin, human resistin, human clusterin, human ANGPTL3, human CTRP5, human IL-33, human leptin, human GPX3, human NMNAT2, human sirtuin 1, human FTO, mouse Nampt, rat Nampt. The assay range is 0.313-20ng NQO1/ml and a detection limit of 100pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).Sample Type:Cell lysatesIntended Use:For the quantitative determination of NQO1 in cells.Sensitivity:100pg/ml Range:0.313-20ng NQO1/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of human NQO1 in cells. A monoclonal antibody specific for NQO1 has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, NQO1 is recognized by the addition of a purified polyclonal antibody specific for NQO1 (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of NQO1 in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlDetection Antibody: 1x12mlLysis Buffer (10X): 1x12mlDetector 100X (HRP conjugated anti-IgG): 1x150ulHuman NQO1 Standard (lyophilized): 40ngHuman NQO1 QC Sample (lyophilized): 1 vialSubstrate Solution (TMB): 1x12mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

產品特點: 針對NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, QR1, DIA4, NMOR1)該產品的特點優勢，歡迎查閱官網提供的產品說明書。

保存建議: 建議收到NQO1, Human, BioAssay ELISA Kit (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, QR1, DIA4, NMOR1)產品后將其置于4°C保存。

其他: Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。