- 中文名稱
Neonatal Cardiomyocyte Isolation System, BioAssay Kit
- 英文名字
- Neonatal Cardiomyocyte Isolation System, BioAssay Kit
- 供應商
- USBiological
- 產品貨號
- N1057
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Neonatal Cardiomyocyte Isolation System has been introduced to provide researchers with a reliable, convenient, and consistent cell isolation system. By utilizing purified rather than crude enzyme preparations, it has been possible to minimize the lot-to-lot variation. In addition, United States Biological use-tests the kits by isolating cardiomyocytes from neonatal rat hearts to assure performance, reliability and consistent yield of viable cells. The kit has been formulated in conjunction with Dr. Ronal MacGregor. The method is based on that described by Toraason, et al. (1988) in which the minced tissue is incubated overnight with purified trypsin at 4°C. As pointed out by Toraason, this step reduces the hands-on time required to harvest cells compared to the time involved in sequential incubations in warm trypsin or collagenase. Also, purified collagenase is utilized to maximize yield and viability. This kit contains sufficient materials for five separate tissue dissociations, each containing up to twelve hearts. For larger or smaller tissue samples prepare proportionate volumes ofreagents at each step and combine them in the same ratio as described in the protocol.Kit Components:N1057-01: Hank's Buffer, 1x500ml Sterile calcium- and magnesium-free Hank's Balanced Salt Solution, pH 7.4. The solution is used for reconstituting N1057-02 Trypsin and N1057-03 Inhibitor in addition to serving as the medium for the dissociation.N1057-02: Trypsin Vials, 5x1000ug, 3X crystallized, dialyzed against 1mM HCl, filtered through 0.22um pore size membrane, and lyophilized. Reconstitute with 2ml N1057-01 Hank's Buffer and swirl gently to dissolve contents. Store at 4°C.N1057-03: Inhibitor Vials, 5x2000ug Supplied as a 0.22um membrane filtered, lyophilized powder. Reconstitute with 1ml N1057-01 Hank's Buffer and swirl gently to dissolve contents. Store at 4°C.N1057-04: Collagenase Vials 5x1500 Units Supplied as a 0.22um membrane filtered, lyophilized powder which has been chromatographically purified. It contains <50 caseinase units/mg and is composed of two separable but very similar collagenases. Reconstitute with 5ml prepared N1057-05 L-15 Media Powder and swirl gently to dissolve contents. Store at 4°C.N1057-05: L-15 Media Powder, 1x1L Reconstitute entire contents of pouch by cutting open top of envelope and pouring contents into beaker containing 800ml of cell culture grade water. Rinse pouch 2-3 times with additional 100ml. Bring total volume to 1 liter and filter through a 0.22um filter.N1057-06: Cell Strainers (Falcon), 5 and a card correlating phenol red color with pH for checking the pH of balanced salt solution and culture medium.Storage and Stability:May be stored at 4°C. Stable for 6 months.
- 產品特點
- 針對Neonatal Cardiomyocyte Isolation System, BioAssay Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Neonatal Cardiomyocyte Isolation System, BioAssay Kit產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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