- 中文名稱
MCAF, Human (Monocyte Chemotactic Activating Factor) BioAssay ELISA Kit
- 英文名字
- MCAF, Human (Monocyte Chemotactic Activating Factor) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- M2690-17
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Monocyte chemotactic and activating factor (MCAF), also known as monocyte chemotactic protein 1 (MCP-1), lymphocyte–derived chemotactic factor (LDCF), and glioma-derived chemotactic factor (GDF), is a recently-identified chemotactic cytokine for monocytes. cDNA cloning and structural analysis has revealed that this 76-amino acid polypeptide with a predicted molecular mass of 8,700 daltons belongs to a family of structurally-related low molecular weight proteins characterized by four conserved cysteine residues designated C-C family or intercrine beta family (1,2,3).This MCAF ELISA is a 2.5 hour solid-phase immunoassay readily applicable to measure MCAF levels in serum, plasma, cell culture supernatant and other biological fluids in the range of 0-1600pg/ml. It has shown no crossreactivity with various other C-C and C-X-C chemokines, IL-8 superfamily proteins. This MCAF ELISA is expected to be effectively used for futher investigation into the relationship between MCAF and various diseases.This MCAF enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for MCAF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. MCAF if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound MCAF and other components of the sample. In order to quantify the amount of MCAF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for MCAF is added to each well to sandwich the MCAF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MCAF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.In order to measure the concentration of MCAF in the samples, this kit contains two calibration diluents (Calibrator Diluent 1 for serum/plasma testing and Calibrator Diluent 2 for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus MCAF concentration (pg/ml). The concentration of MCAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: < 4pg/mlRange: 0-1600pg/mlKit Components:1. MCAF Microtiter Plate, 1x96 wells2. MCAF Conjugate, 1x15ml3. MCAF Standard, 2x1 vial 4. Calibrator Diluent 1, 1x22ml5. Calibrator Diluent 2, 1x22ml6. Wash Buffer (20X), 1x60ml7. Substrate A, 1x11ml8. Substrate , 1x11ml9. Stop Solution, 2N Sulphuric Acid (H2SO4). Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產品特點
- 針對MCAF, Human (Monocyte Chemotactic Activating Factor) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到MCAF, Human (Monocyte Chemotactic Activating Factor) BioAssay ELISA Kit產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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