- 中文名稱
小鼠來源單克隆抗體：Amyloid beta peptide（A-beta 40/42），【MOAB-2】 - Biotinylated
- 英文名字
- Mouse monoclonal antibody to Amyloid beta peptide (A-beta 40/42),【MOAB-2] - Biotinylated
- 供應商
- Biosensis
- 產品貨號
- M-1742-50-B
- 產品報價
- ￥詢價/50ug
- 產品說明書
- 點擊查看
- 購買方式
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- 產品新聞
- 背景資料
- Biosensis品牌專注于神經科學領域的抗體和試劑的開發，在神經科學領域屬于全球領航者，被廣泛用于阿爾茨海默氏癥（AD）、帕金森氏癥（PD）和肌萎縮側索硬化（ALS）疾病，以及自噬和代謝應激障礙的研究。武漢艾美捷科技作為Biosensis品牌中國區域總代理，提供Biosensis品牌特色的組織染色方案：FJC退化神經元染色試劑盒、病理髓鞘染色試劑盒、淀粉樣斑塊染色試劑等。武漢艾美捷科技擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊，可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 應用類型
- WB; IH, Frozen; IF,Frozen; IH(P), Paraffin embedded; IF; IP; ELISA
The biotinylated MOAB-2 antibody has been tested by IHC (1:500 - 1:2,000 dilution) and is also expected to work in applications validated for the unlabelled antibody (M-1586-100) at same or higher dilutions: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IHC(P), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA.
Western Blotting:
MOAB-2 has been tested in WB using purified synthetic beta-amyloid preparations and from transgenic mouse brain formic acid extracts (see Figure 1). Formic acid extraction/concentration is required for western blot detection from extracts. Suggested dilution of 1:2000-1:5,000 for WB, standard ECL detection systems.
Tissue samples for the detection of beta-amyloid should be prepared as detailed in Youmans KL et al., 2011 (Journal of Neuroscience Methods 196: 51-59) for best results. Detection of beta-amyloid 40/42 in direct westerns can be difficult; Dot-blots of prepared samples are recommended as detailed in Youmans KL et al., 2012.
Immunohistochemistry:
Suggested dilution for biotinylated MOAB-2 in IHC is 1:500-1:2,000. Fresh frozen, 4% paraformaldehyde fixed frozen, or formalin fixed paraffin embedded tissues are all suitable. Antigen retrieval is required in fixed tissues for optimal staining.
Antibody was tested on 4% paraformaldehyde/0.1% glutaraldehyde fixed frozen tissue from 3xTg and 5xFAD mice. MOAB-2 antibody detects intraneuronal and extracellular beta-amyloid in IHC and does not detect APP (Youmans KL et al., 2012).
The antibody also reacts with archival formalin-fixed, paraffin-embedded tissue samples with antigen Heat Induced Epitope Retrieval (HIER). Recommended buffer for HIER is citrate, pH 6.0. Signal was weak without antigen retrieval. Immunoreactivity was observed in intraneural-amyloid deposition (plaque) in Alzheimer's brain. MOAB-2 was found to be extremely clean and with an excellent signal to noise ratio with no neuro-cellular diffusive staining.
In addition, MOAB-2 demonstrated no significant differences in A-beta detection using paraffin fixed, free-floating sections (Youmans KL et al., 2012). Formic acid (FA) treatment resulted in optimal detection of both intraneuronal and extracellular A-beta compared to without FA (incubated in 88% FA 8 min, Youmans KL et al., 2012). Free floating tissue sections were permeabilized in TBS containing 0.25% Triton X-100 (TBSX; 3 x 10 min), blocked with 3% horse serum in TBSX (3 x 10 min) followed by 1% horse serum in TBSX (2 x10 min) and incubated with appropriate primary antibodies diluted in TBSX containing 1% horse serum overnight. See Youmans KL et al., 2012, for full IHC(P) protocol and method details.
Immunofluorescence:
For IF, suggested dilution is 1:100-1:500. The antibody was tested on 4% PFA fixed frozen tissue. Fixed tissues were washed in TBS (3 x 10 min), then incubated in 88% FA (8 min), and then permeabilized in TBSX (3 x 10 min), and blocked in TBSX containing 5% bovine serum albumin (BSA; 1 hr). Sections were subsequently incubated with appropriate primary antibodies diluted in TBSX containing 2% BSA overnight on an oscillatory rotator. Detection was via fluorescently labelled absorbed secondary antibodies (Youmans KL et al., 2012).
Immunoprecipitation:
For IP, the suggested dilution is 1:200 to 1:1,000 for labelled beta-amyloid using SA-coated beads as the capture vehicle, similar to the protocols employed by Youmans KL et al., 2012.
ELISA:
In an ELISA, a dilution of 1:50-1:1,000 is suggested. The antibody has been tested in ELISAs on synthetic beta-amyloid and tissue homogenates from beta-amyloid-Tg mice.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user for all applications. Dilutions provided are only meant to serve as a basic guide.
- 免疫原
- Recombinant human amyloid beta protein 42 (AB42): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
- 來源宿主
- 小鼠
- 反應性
- 人, 大鼠, other species not yet tested. By Dot Blot, MOAB-2 detected 大鼠 AB40, 人 AB40, albeit with less affinity than for AB42 (Youmans KL et al., 2012).
- 保存建議
- 廠家建議常溫運輸?？贵w溶解之后，建議分裝保存于-20℃或-70℃。在2-8 ℃可保存1周（避光，使用無菌器材）。按照1:1體積比添加最高純度的甘油，可增加抗體的穩定性。請避免反復凍融。不適用時，應保持蓋子緊閉并避光保存。
- 其他
- Biosensis專注于神經科學領域的抗體和試劑的開發，特別是神經營養因子和神經營養因子受體。 近30年來，Biosensis一直是該領域的全球領航者和OEM供應商。除神經營養因子，我們的神經科學產品組合還被廣泛用于神經退行性疾病、神經發育和神經代謝的研究。重點研究領域包括阿爾茨海默氏癥（AD）、帕金森氏癥（PD）和肌萎縮側索硬化（ALS）疾病，以及自噬和代謝應激障礙，包括肥胖、代謝綜合征的研究、神經免疫學和炎癥。Biosensis的產品系列不僅包括持續增長的神經科學研究抗體系列，還包括200多種定量研究ELISAs試劑盒（1板或2板，自由選擇），組織染色（退化神經元染色試劑盒、病理髓鞘染色試劑盒、淀粉樣斑塊染色試劑等）和細胞可視化試劑（染色）以及針對神經科學和細胞疾病研究的純化的蛋白質。我們的抗體和試劑已被廣泛應用于各種技術，包括蛋白質印跡，免疫組織化學，流式分析，共聚焦顯微鏡實時成像，生物抑制和細胞培養以及克隆。
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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