- 中文名稱
MOAB-2 小鼠來源單克隆抗體：Amyloid beta peptide（A beta 40/42）， purified
- 英文名字
- MOAB-2 Mouse Monoclonal antibody to Amyloid beta peptide (A beta 40/42), purified
- 供應商
- Biosensis
- 產品貨號
- M-1586-100
- 產品報價
- ￥詢價/100ug
- 產品說明書
- 點擊查看
- 購買方式
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- 產品新聞
- 背景資料
- Biosensis品牌專注于神經科學領域的抗體和試劑的開發，在神經科學領域屬于全球領航者，被廣泛用于阿爾茨海默氏癥（AD）、帕金森氏癥（PD）和肌萎縮側索硬化（ALS）疾病，以及自噬和代謝應激障礙的研究。武漢艾美捷科技作為Biosensis品牌中國區域總代理，提供Biosensis品牌特色的組織染色方案：FJC退化神經元染色試劑盒、病理髓鞘染色試劑盒、淀粉樣斑塊染色試劑等。武漢艾美捷科技擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊，可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 應用類型
- WB; IHC, Frozen; IH(P), Paraffin embedded; IF, Frozen; IF(P), Paraffin embedded; IC; IP; FC; ELISA
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry/paraffin embedded IH(P), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA.
Antibody has been tested in WB using purified synthetic beta-amyloid preparations and from transgenic mouse brain formic acid extracts (see figure 1). Formic acid extraction/concentration is required for western blot detection from extracts. MOAB-2 antibody is specific for beta-amyloid and does not detect APP. Suggested dilution of 1:2000-1:5,000 for WB, standard ECL detection systems.
Tissue samples for the detection of beta-amyloid should be prepared as detailed in K.L. Youmans et al. {Journal of Neuroscience Methods 196 (2011) 51-59} for best results. Detection of beta-amyloid 40/42 in direct westerns can be difficult; Dot-blots of prepared samples are recommended as detailed in Youmans. KL et al 2012.
IR or fluorescent detection systems not yet tested, they but are expected to work well with higher primary antibody dilutions because of the increased sensitivity of the detection methods.
Suggested dilutions for IHC are 1:50-1:1,000. Fresh frozen, 4% paraformaldehyde fixed frozen, or formalin fixed paraffin embedded tissues are all suitable. Optimal dilutions must be determined by the end user. Antigen retrieval is required in fixed tissues for optimal staining.
Antibody was tested on 4% paraformaldehyde/0.1% glutaraldehyde fixed frozen tissue from 3xTg and 5xFAD mice. MOAB-2 antibody detects intraneuronal and extracellular beta-amyloid in IHC and does not detect APP {Youmans KL et al 2012}.
The antibody also reacts with archival formalin-fixed, paraffin-embedded tissue samples with antigen Heat Induced Epitope Retrieval (HIER): Recommended Citrate, pH 6.0 buffer for HIER. Signal was weak without antigen retrieval. Immunoreactively was expressed in intraneural-amyloid deposition (plaque) in Alzheimer's brain. MoAB-2 was found to be extremely clean and with an excellent signal to noise ratio with no neuro-cellular diffusive staining.
In addition MOAB-2 demonstrated no significant differences in A-beta detection using paraffin fixed, free-floating sections {Youmans KL et al 2012}. Formic acid (FA) treatment resulted in optimal detection of both intraneuronal and extracellular A-beta compared to without FA (incubated in 88% FA 8 min, Youmans KL et al 2012). Free floating tissue sections were permeabilized in TBS containing 0.25% Triton X-100 (TBSX; 3 x 10 min), blocked with 3% horse serum in TBSX (3 x 10 min) followed by 1% horse serum in TBSX (2 x10 min) and incubated with appropriate primary antibodies diluted in TBSX containing 1% horse serum overnight. See Youmans KL et al 2012 for full IH(P) protocol and method details.
For IF, suggested dilution is 1:100-1:500. The antibody was tested on 4% PFA fixed frozen tissue. Fixed tissues were washed in TBS (3 x 10 min), then incubated in 88% FA (8 min), and then permeabilized in TBSX (3 x 10 min), and blocked in TBSX containing 5% bovine serum albumin (BSA; 1 hr). Sections were subsequently incubated with appropriate primary antibodies diluted in TBSX containing 2% BSA overnight on an oscillatory rotator. Detection was via fluorescently labelled absorbed secondary antibodies {Youmans KL et al 2012}.
For IP, the suggested dilution is 1:200 to 1:1,000 for labeled beta-amyloid using Protein A/G conjugated beads as the capture vehicle {Youmans KL et al 2012}.
In an ELISA, a dilution of 1:50-1:1000 is suggested. The antibody has been tested in ELISAs on synthetic beta-amyloid and tissue homogenates from beta-amyloid-Tg mice. Biosensis recommends optimal dilutions/concentrations should be determined by the end user for all applications. Dilutions provided are only meant to serve as a basic guide.
- 免疫原
- Recombinant human amyloid beta protein 42 (AB42): DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
- 來源宿主
- 小鼠
- 反應性
- 人, 大鼠, other species not yet tested.By Dot blot, MOAB-2 detected 大鼠 AB40, 人 AB40, albeit with less affinity than for AB42. {Youmans. KL et al 2012}
- 保存建議
- 廠家建議常溫運輸?？贵w溶解之后，建議分裝保存于-20℃或-70℃。在4℃可保存1周（避光）。使用無菌操作，按照1:1體積比添加最高純度的甘油，可增加抗體的穩定性。請避免反復凍融。不適用時，應保持蓋子緊閉并避光保存。
- 其他
- Biosensis專注于神經科學領域的抗體和試劑的開發，特別是神經營養因子和神經營養因子受體。 近30年來，Biosensis一直是該領域的全球領航者和OEM供應商。除神經營養因子，我們的神經科學產品組合還被廣泛用于神經退行性疾病、神經發育和神經代謝的研究。重點研究領域包括阿爾茨海默氏癥（AD）、帕金森氏癥（PD）和肌萎縮側索硬化（ALS）疾病，以及自噬和代謝應激障礙，包括肥胖、代謝綜合征的研究、神經免疫學和炎癥。Biosensis的產品系列不僅包括持續增長的神經科學研究抗體系列，還包括200多種定量研究ELISAs試劑盒（1板或2板，自由選擇），組織染色（退化神經元染色試劑盒、病理髓鞘染色試劑盒、淀粉樣斑塊染色試劑等）和細胞可視化試劑（染色）以及針對神經科學和細胞疾病研究的純化的蛋白質。我們的抗體和試劑已被廣泛應用于各種技術，包括蛋白質印跡，免疫組織化學，流式分析，共聚焦顯微鏡實時成像，生物抑制和細胞培養以及克隆。
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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