- 中文名稱
Leukotriene C4 (LTC4) BioAssay ELISA Kit
- 英文名字
- Leukotriene C4 (LTC4) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- L2047-10
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Leukotriene C4 (LTC4) is a potent mediator of immediate hypersensitivity, bronchoconstriction, smooth muscle contraction, increased vascular permeability and epithelial mucous secretion. It is derived from arachidonic acid through the 5-lipoxygenase pathway. Arachidonic acid is first converted to 5-hydroperoxy derivative which is then transformed to Leukotriene A4 (LTA4). Enzymatic addition of glutathione to LTA4 produces LTC4.Principle of the Assay:This is an ELISA (Enzyme-Linked Immunosorbent Assay) for the quantitative analysis of Leukotriene C4 levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Leukotriene C4 in the sample for a limited number of binding sites. First, the sample or standard solution is added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed, removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes. Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of Leukotriene C4 in the sample or standard. For example, the absence of Leukotriene C4 in the sample will result in a bright blue color, whereas the presence of Leukotriene C4 will result in decreased or no color development.Kit Components:L2047-10A: ELISA Buffer: 1x30ml. To be used to dilute enzyme conjugate and Leukotriene C4 standardsL2047-10B: Wash Buffer 10X: 1x20ml. To be diluted 10X with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.L2047-10C: Substrate: 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.L2047-10D: Extraction Buffer 5X: 1x30ml. To be diluted 5X with deionized water. This is used for diluting extracted and non-extracted samples..*L2047-10E: Leukotriene C4 (HRP): 2x75ul. Lyophilized LTC4 HRP conjugate. Reconstitute one vial with 75ul of deionized water for a 50X stock solution.L2047-10F: Leukotriene Standard C4: 1x100ul Leukotriene C4 standard at the concentration of 1ug/ml.L2047-10G: Leukotriene C4 Microtiter Plate: 1x96 well MaxiSorp Nunc microplate with anti-LTC4 rabbit antibody precoated on each well. The plate is ready to use as is. Do not wash.Storage and Stability:Store *L2047-10E powder at -20°C liquid at 4°C. Do not freeze. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Leukotriene C4 (LTC4) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Leukotriene C4 (LTC4) BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
-
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
-
