- 中文名稱
【干冰】Histone Targeted ChIP-seq Kit - KOA0881
- 英文名字
- 【干冰】Histone Targeted ChIP-seq Kit - KOA0881
- 供應商
- Rockland
- 產品貨號
- KOA0881
- 產品報價
- ￥詢價/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- Association between proteins and DNA is a major mechanism in many vital cellular functions such as gene transcription and epigenetic silencing. It is crucial to understand these interactions and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is a technique to analyze the association of proteins with specific genomic regions in intact cells. ChIP can be used to study changes in epigenetic signatures, chromatin remodelling and transcription regulator recruitment to specific genomic sites.
In ChIP, living cells are first fixed with a reversible cross-linking agent to stabilize protein-DNA interactions. The most widely used reagent to fix cells is formaldehyde which generates covalent bonds between amino or imino groups of proteins and nucleic acids. Formaldehyde treatment crosslinks both DNA-protein as well as protein-protein complexes.
Following cross-linking, chromatin needs to be sheared very efficiently into homogeneous small fragments that can subsequently be used in immunoprecipitation (IP). After fragmentation, the sheared chromatin is precipitated with a specific antibody (AB) directed against the protein of interest. The chromatin-AB complex is isolated using magnetic beads. Finally, the precipitated DNA fragments are released from the AB, and analyzed. Enrichment of specific sequences in the precipitated (IP’d) DNA indicates that these sequences were associated with the protein of interest in vivo. Analyzis of specific regions can be performed by quantitative polymerase chain reaction (qPCR). In recent years, ChIP combined with high-throughput Next-Generation sequencing (ChIP-seq) has become the gold standard for whole-genome mapping of protein-DNA interactions.
- 產品描述
- The different steps of the ChIP assay are cell fixation (cross-linking), chromatin shearing, immunoprecipitation, reverse cross-linking followed by DNA purification and analysis of the immunoprecipitated DNA. Suitable for 24 reactions.
- 產品特點
- 該【干冰】Histone Targeted ChIP-seq Kit - KOA0881的詳細信息查看Rockland提供的產品說明書。
- 保存建議
- See kit insert for components stored at 2-8°C and -20°C. Do not freeze Protein A and magnetic beads.
- 其他
- Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。
-
- 注意
-
該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
-
