Oxphos? Cell Survival Assay Kit - KLD-002-Oxphos? Cell Survival Assay Kit - KLD-002代理/參數/廠家-免疫學試劑盒-艾美捷科技有限公司

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背景資料: OxPhos? is a medium based metabolic probe that can measure glutathione recycling capacity/glutathione function and glucose dependent antioxidant capacity of cells in tissue culture and whole blood. Oxphos? is based on the ability of mammalian cells to rapidly and efficiently convert hydroxyethyl disulfide (HEDS) into mercaptoethanol (ME) through a bioreduction mechanism. Bioconversion of HEDS to ME relies on the activity of the oxidative pentose phosphate cycle (OPPC). OxPhos? measures ME in the extracellular medium without the need for cellular lysis and extraction methods. Cell media is used in the assay, avoiding the need to lyse cells and thereby saving time and cost while preserving the ability to perform other cellular tests in the same culture system. OxPhos? may have multiple applications in aging, oxidative stress, antioxidant screening, chemotherapy response and toxicology.

產品描述: Oxphos? gives a linear response for mammalian cells (0; 100,000; 200,000; 400,000; 600,000; and 800,000) plated in 1mL growth medium in a six well plate with up to 15% fetal bovine serum and measured 20 hours after plating. It also gives a linear response for human blood (0, 10, 20, 40, 60, 80, 100 μL) suspended in a total volume of 200 μL saline in a microfuge tube and incubated with 10 μL of reagent 6 for 2hrs. Oxphos? contains enough reagents for 100 assays using a 6-well plate.

保存建議: Store kit at 2-8° C prior to opening. See kit insert for complete instructions.

其他: Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。