The FLAG? epitope is a small but highly immunogenic peptide DYKDDDDK (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) which allows fusion proteins to retain their original conformation and function. The hydrophilic character of this sequence increases the likelihood that it will be located on the surface of FLAG? -tagged recombinant proteins for reaction with anti-DYKDDDDK (FLAG?) antibodies. The anti-DYKDDDDK (FLAG?) antibody included in this Western blot kit detects FLAG? -tagged recombinant proteins fused with FLAG? tag either at the amino-terminus or carboxy-terminus of the protein.
產品描述
Use Rockland Immuno-chemicals’ Anti-DYKDDDDK (FLAG?) Chemiluminescent Kit for Western Blotting for detection of FLAG? -tagged recombinant proteins by Western blot. This kit is useful for both ''Western blotting'' and ''dot blotting'' methods. After protein separation and transfer, the membrane is probed with Anti-DYKDDDDK (FLAG?). Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
產品特點
該Chemiluminescent Western Blot Kit: DYKDDDDK (FLAG?) Chemiluminescent Western Blotting Kit - KCA383的詳細信息查看Rockland提供的產品說明書。
