TrueBlot? Protein G Magnetic Beads IP/Co-IP Kit - KBA-PG00-18-TrueBlot? Protein G Magnetic Beads IP/Co-IP Kit - KBA-PG00-18代理/參數/廠家-免疫學試劑盒-艾美捷科技有限公司

PRODUCT CENTER

免疫學試劑盒

產品中心

當前位置：首頁 > 產品中心 > 免疫學產品 > 免疫學試劑盒

背景資料: TrueBlot? Protein G Magnetic Beads IP/Co-IP Kit allows highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of an antigen using smaller amounts of antibody (typically 1-10 μg). The kit is designed for immunoprecipitation of proteins, protein complexes, protein-nucleic acid complexes and other antigens. The specific antibody is added to the sample to form an immune complex. The immune complex is then bound to Protein G Magnetic beads. The complex is washed to remove unbound material and the bound immune complex is then eluted from the beads using low-pH elution buffer. Alternatively, the sample buffer is used for dissociation using denaturing conditions or for down-stream process for SDS-PAGE analysis. The kit includes Protein G magnetic beads for high capture efficiencies and optimized buffers for high yield of target antigen. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL. The kit contains magnetic beads, Binding/Wash buffer, Elution buffer, and Neutralization buffer sufficient for 20 samples or tests. (See protocol for details). The beads are removed from the solution using a magnetic separator.

產品描述: TrueBlot? Protein G magnetic beads IP/Co-IP kit can be used for immunoprecipitation (IP) and Co-IP experiments as well as for antibody purification. For IP, target specific antibody is incubated with cell lysate. Protein G magnetic beads are then incubated with antigen-antibody complex at room temperature, washed and then eluted using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting. For antibody purification, Protein G magnetic beads are incubated with the antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually.

產品特點: 該TrueBlot? Protein G Magnetic Beads IP/Co-IP Kit - KBA-PG00-18的詳細信息查看Rockland提供的產品說明書。

保存建議: The Protein G Magnetic Beads should be stored in the refrigerator (2-8 °C). The reagent must be allowed to reach room temperature (20-25 °C) before use and may be used until the expiration date. Do not freeze, dry, or centrifuge the beads as they may result in loss of binding activity and aggregation. See kit insert for storage components.

其他: Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。