B-Phycoerythrin Labeling Kit-SH is primarily used for the preparation of B-phycoerythrin-labeled antibody for immunostaining and cellular proteins for tracing· SH-Reactive B-Phycoerythrin, a component of this kit, has maleimide group that reacts with sulfhydryl groups (SH) of reduced IgG or other proteins· This kit contains all of the necessary reagents for the labeling· Reducing Agent in this kit produces free sulfhydryl groups in the IgG molecule without loss of antibody affinity· The labeling process is simple· After the reducing reaction, add SH-Reactive BPhycoerythrin to IgG solution on the membrane of Filtration Tube, and incubate at 37°C for 1 hour· The maximum excitation and emission wavelengths of the B-phycoerythrin-labeled proteins are 564 nm and 575 nm, respectively·
