總磷酸二酯酶活性測定試劑盒（熒光法）-Total Phosphodiesterase Activity Assay Kit (Fluorometric)代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

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產品描述: Phosphodiesterase (PDE) is a family of enzymes that cleave a phosphodiester bond. These include cyclic nucleotide phosphodiesterases, phospholipase C and D, RNases, DNases and some restriction endonucleases. Phosphodiesterases are important enzymes used for a variety of biological functions including metabolism of second messenger molecules like cAMP / cGMP (therefore, regulation of their intracellular levels). PDE activity is responsible for degradation of mRNA after it had been translated to protein and of DNA during apoptosis. Some PDE isoforms such as PDE 5 and PDE 11 are therapeutic targets because of their role in the cardiovascular system and tumor formation respectively. BioVision’s PDE assay kit is a fluorometric plate-based assay for kinetic measurement of total phosphodiesterase activity. It is based on the cleavage of a coumarin based synthetic substrate by phosphodiesterases to generate a product, which fluoresces at Ex/Em= 370/450 nm. It does not rely on the quantification of phosphate released by phosphodiesterase action, like other traditional methods currently in use. Therefore, sample background is negligible, and does not interfere with the assay. The substrate is specific for phosphodiesterases and is not cleaved by enzymes that act on carboxyl esters or by phosphatases. This assay can detect as low as 0.25 μU of total PDE.

產品特點: ? Simple, highly sensitive, high-throughput compatible ? Detection and quantificiation of total phosphodiesterase activity in biological samples ? This assay has a detection sensitivity as low as 0.25 μU.

其他: BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.