- 中文名稱
EZClick?O-GlcNAc修飾糖蛋白測定試劑盒（FACS /顯微鏡，綠色熒光）
- 英文名字
- EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
- 供應商
- Biovision
- 產品貨號
- K714-100
- 產品報價
- ￥詢價/100assays
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.
- 產品新聞
- 背景資料
- 待更新
- 產品描述
- Glycans are vital components of glycoproteins, glycolipids, and proteoglycans in all domains of life. Glycoproteins are grouped by the type of carbohydrate and amino acid linkage site. N-linked glycosylation is a modification of asparagine, whereas O-linked glycosylation occurs through the hydroxyl group of serine and threonine residues. Glycosylation occurs co- or post-translationally on >50% of eukaryotic proteins resulting in membrane-associated, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Modification by O-linked-N-acetyl glucosamine (O-GlcNAc) has rapidly emerged as a major cellular signaling mechanism with number of modified targets similar to protein phosphorylation. Many oncogenes and tumor suppressors are regulated by O-GlcNAcylation. O-GlcNAc modification is ubiquitous among eukaryotes, from yeast to humans and its modifying enzymes have been well characterized. O-GlcNAc modified nuclear and cytosolic targets include: transcription factors, signaling proteins, metabolic enzymes, mitochondrial trafficking, cell cycle regulation, glucose homeostasis. O-GlcNAc glycosylation is implicated in normal brain functions, etiology of neurodegeneration, type II diabetes, and pathways involved in morphogenesis and virulence factors of microbes and plant host cells. Since glycoproteins are not directly encoded in the genome, methods of characterization and analyses of glycoproteins are of great interest. Thus BioVision offers EZClickTM O-GlcNAc Modified Glycoprotein Assay Kit, a highly specific, simple and robust method for labeling and detection of O-GlcNAc-glycosylated proteins within cells. We use a modified glucosamine precursor that is fed directly into the cells, processed by the hexosamine pathway and incorporated into the proteins. Followed by click reaction with alkyne-containing dye, this system offers a powerful method for imaging the localization, trafficking, and dynamics of glycans, or detection by FACS for quantitative studies. Labeled Glycoproteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-alkyne or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format
- 產品特點
- Simple, fast, does not require lengthy incubation times
- 保存建議
- -20
- 其他
- BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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