- 中文名稱
UGT活性測定/配體篩選試劑盒（熒光法）
- 英文名字
- UGT Activity Assay / Ligand Screening Kit (Fluorometric)
- 供應商
- Biovision
- 產品貨號
- K692-100
- 產品報價
- ￥詢價/100assays
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.
- 產品新聞
- 背景資料
- 待更新
- 產品描述
- Uridine diphospho-glucuronosyltransferases (UGTs, EC 2.4.1.17) are a superfamily of microsomal membrane-bound glycosyltransferase enzymes. UGTs are responsible for the vast majority of Phase II biotransformation (conjugation) reactions, in which a hydrophilic moiety is attached to small molecule drugs and other xenobiotics to facilitate their rapid excretion from the body. UGTs are predominantly expressed in the liver, intestine and kidneys, where they catalyze the addition of a glucuronic acid moiety from the nucleotide sugar uridine-5’-diphospho-α-D-glucuronic acid (UDPGA) to nucleophilic hydroxyl, sulfhydryl or amino groups of pharmacologically active drugs, Phase I drug metabolites and certain endogenous substrates such as steroid hormones, bile acids and bilirubin. Isozymes of the UGT family have extremely broad, often overlapping substrate specificities and tend to exhibit modest affinity for substrates but high catalytic turnover rates. UGTs also contribute to ‘first-pass’ presystemic drug metabolism, which dramatically reduces the oral bioavailability of a number of drugs. BioVision’s UGT Activity Assay / Ligand Screening Kit enables rapid measurement of native or recombinant UGT activity in biological samples such as liver microsomes and can also be used to assess the effect of drugs and other novel compounds on UGT activity. The assay utilizes a highly fluorescent UGT substrate with a large Stokes shift (Ex/Em = 415/502 nm) that allows determination of UGT activity by tracking the drop in fluorescence emission as the substrate is converted into a non-fluorescent glucuronide. The multi-isozyme substrate is glucuronidated by virtually all of the pharmacologically-relevant mammalian UGT1A and UGT2B enzymes. UGT specific activity is calculated by comparing the fluorescence loss versus a control reaction performed in the absence of the required cofactor UDPGA. The kit includes the pore-forming peptide antibiotic Alamethicin, which allows the UGT Substrate and UDPGA to rapidly diffuse across lipid membranes to access the UGT active site located in the lumen of microsomes. For verification of modulation of UGT activity by test ligands, diclofenac, a competitive inhibitor of most human and rodent UGT isozymes, is also included. The assay is highly sensitive, simple to perform and high-throughput adaptable. This assay can detect less than 0.1 mU UGT activity in biological samples. The kit contains a complete set of reagents sufficient for performing 100 reactions at a 100 μl reaction volume
- 產品特點
- ? Easy-to-use
? Non-radioactive
? Accurately measure UGT activity in microsomes, lysates and recombinant UGT preparations
- 保存建議
- -20
- 其他
- BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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