- 中文名稱
可溶性膠原蛋白檢測試劑盒（熒光法）
- 英文名字
- Soluble Collagen Assay Kit (Fluorometric)
- 供應商
- Biovision
- 產品貨號
- K532-100
- 產品報價
- ￥詢價/100assays
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.
- 產品新聞
- 背景資料
- 待更新
- 產品描述
- Collagen is the most abundant protein in mammals, accounting for nearly 30% of body protein content. Collagen is an extracellular scaffolding protein found in skin, tendons, bone, cartilage, ligaments and muscles, amongst other tissues. There are more than twenty different characterized types of collagen, but the fibrilous types I, II and III comprise more than 90% of the total collagen in mammals. Collagen has a triple helical structure, consisting of three ‘preprocollagen’ strands with a repeating glycine- and proline-rich tripeptide sequence (Gly-Pro-X) and small end-cap domains called registration peptides. Nascent preprocollagen strands undergo enzymatic post-translational hydroxylation at proline and lysine residues prior to secretion to the extracellular milieu via the golgi apparatus. Once outside the cell, the dangling registration peptides are cleaved off, forming tropocollagen, the monomeric precursor to mature collagen fibers. While mature collagen exists as an insoluble web of extensively crosslinked fibrils, tropocollagen is soluble in dilute acidic solutions. Many diseases are believed to affect collagen synthesis, fibrilization and turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. BioVision’s Soluble Collagen Assay Kit allows for quantification of newly synthesized (acid-soluble) collagen levels in tissues and cultured cell medium. The assay involves extraction of soluble collagen in acetic acid, followed by enzymatic degradation of collagen into glycine-rich oligopeptides, which are quantified using a fluorogenic reagent and developer solution that selectively react with the N-terminal glycine fragments to form a stable fluorescent complex (Ex/Em = 376/468 nm). The assay is more sensitive and selective than dye-binding (Picrosirius Red) soluble collagen assays, is simple to perform and has a linear range from 0.05 – 2 μg collagen per well (2.5 μg/ml to 100 μg/ml in a 20 μl sample volume)
- 產品特點
- ? Simple, fast and convenient assay
? Can detect as low as 0.05 μg of soluble collagen in a variety of samples
? Simple procedure; takes less than ~60 minutes
? Increased sensitivity (Fluorometric Detection)
? PCA-free
- 保存建議
- -20
- 其他
- BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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