- 中文名稱
多藥外排轉運蛋白（MDR1 / P-gp）配體篩選試劑盒
- 英文名字
- Multidrug Efflux Transporter (MDR1/P-gp) Ligand Screening Kit
- 供應商
- Biovision
- 產品貨號
- K507-100
- 產品報價
- ￥詢價/100Assays
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.
- 產品新聞
- 背景資料
- 待更新
- 產品描述
- P-glycoprotein (P-gp, Multidrug Resistance Protein 1 (MDR1), EC 3.6.3.44) is a member of the ATP-binding cassette (ABC) ATPase superfamily of transmembrane transporter proteins. P-gp has an extremely broad substrate specificity and is capable of transporting a vast array of neutral and anionic lipophilic molecules. P-gp strongly affects the oral absorption, tissue distribution and excretion of many drugs and prevents certain lipophilic drugs from penetrating the blood brain barrier. Overexpression of P-gp confers tumor cells with resistance to chemically and pharmacologically distinct chemotherapeutic drugs (such as doxorubicin, vincristine and paclitaxel) by actively pumping them out of cells. Induction of P-gp expression is a frequent cause of treatment failure and tumor-targeted delivery of P-gp inhibitors is being investigated as a strategy for overcoming chemotherapy resistance. BioVision’s MDR1/P-gp Ligand Screening Kit is designed for rapidly screening test compounds for modulation of efflux transporter activity in MDR1-expressing cell lines. The assay uses a lipophilic non-fluorescent P-gp substrate that readily diffuses through the plasma membrane, where it is hydrolyzed to an active fluorophore by cytosolic esterases. The resulting hydrophilic fluorophore is neither membrane permeable nor a substrate for P-gp, hence it remains trapped inside the cell. In MDR1-expressing cell lines, the lipophilic pro-fluorophore is continuously extruded from the cytosol by P-gp, leading to a low intracellular fluorescence. Inhibition of P-gp-mediated efflux by a test compound leads to increased intracellular fluorescence. Specific transporter activity is quantified by comparison with fluorescence accumulated in the presence and absence of a saturating concentration of the included selective P-gp inhibitor. The assay is highly sensitive, has a simple no-wash protocol and is high-throughput adaptable. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.
- 產品特點
- ? Simple method to screen Pg-p inhibitors
? High-throughput adaptable
? Includes Inhibitor Control, Verapamil
- 保存建議
- -20
- 其他
- BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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