- 中文名稱
PPDK（丙酮酸，磷酸二激酶）活性測定試劑盒（熒光/比色法）
- 英文名字
- PPDK (Pyruvate, phosphate dikinase) Activity Assay Kit (Fluorometric/Colorimetric)
- 供應商
- Biovision
- 產品貨號
- K456-100
- 產品報價
- ￥詢價/100assays
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.
- 產品新聞
- 背景資料
- 待更新
- 產品描述
- Pyruvate, phosphate dikinase, PPDK, (E.C. 2.7.9.1) is found in microorganisms and plants where it catalyzes reversible interconversion of AMP, PPi and phosphoenolpyruvate (PEP) to ATP, Pi and pyruvate monophosphate. PPDK plays an important role in plant photosynthesis and glycolytic pathway of endobiotic protozoa and bacteria where pyruvate, phosphate dikinase (PK) is absent; it functions in the process of ATP biosynthesis. In plants, PPDK is a pivotal regulator of the C4 photosynthetic cycle and the key limiting factor for maintaining photosynthesis at low temperature. It catalyzes the regeneration of PEP as the primary acceptor of atmospheric CO2 from pyruvate, ATP and phosphate. In addition, PPDK is also present in C3 leaves where it confers a similar light/dark regulation in chloroplasts, and in wheat leaves in an amount comparable to that found in seeds. Since PPDK is not found in vertebrates, finding its inhibitors may be useful for treatment of infections caused by anaerobic protists that depend on pyrophosphate-dependent glycolysis. Specific inhibition of PPDK is expected to inhibit Giardia and other anaerobic protists such as Entamoeba histolytica and Trichomonas vaginalis without harming their mammalian hosts. BioVision’s Pyruvate, phosphate dikinase, (PPDK) Assay Kit provides a simple and rapid test for measuring specific activity of PPDK in bacterial, protozoan and plant lysates as well as protein preps. We utilize the ability of PPDK to interconvert the substrate into intermediate product detected by proprietary enzyme mix and probe. Generated stable product can be quantified by either colorimetric or fluorometric readout. The assay is simple to perform, high-throughput adaptable and a fluorometric reaction can detect as low as of 0.006 U of PPDK activity in a single well. We provide sufficient reagents for 100 fluorometric or colorimetric assays.
- 產品特點
- ? Simple procedure; takes ~ less than 60 minutes
? Fast and convenient
- 保存建議
- -20
- 其他
- BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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