- 中文名稱
EZClick?唾液酸（ManAz）修飾糖蛋白測定試劑盒（FACS /顯微鏡，綠色熒光）
- 英文名字
- EZClick? Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
- 供應商
- Biovision
- 產品貨號
- K441-100
- 產品報價
- ￥詢價/100assays
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.
- 產品新聞
- 背景資料
- 待更新
- 產品描述
- Glycans are vital components of glycoproteins, glycolipids, and proteoglycans in all domains of life. Glycosylation occurs co- or post-translationally on >50% of eukaryotic proteins resulting in membrane-associated, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Intracellular glycans mediate protein folding, stability, and trafficking while at the cell surface, they participate in recognition, cell-cell interactions involved in adhesion, migration, and embryonic development; host-pathogen interactions critical for bacterial and viral infections; and initiation of immune response. Aberrant glycosylation profiles correlate with inflammation and are universal feature of cancer, with sialic acids playing an especially prominent role as tumor associated carbohydrate antigens (TACAs). Altered sialylation of tumor cell surfaces is associated with several critical malignant properties that include invasiveness and metastatic potential suggesting its implication in clinical diagnosis. Since glycoproteins are not directly encoded in the genome, methods of characterization and analyses of glycoproteins are of great interest. Thus BioVision offers EZClickTM Sialic Acid (ManAz) Modified Glycoprotein Assay Kit, a highly specific, simple and robust method for labeling and detection of N-linked glycosylation of cell surface proteins. We use a modified mannosamine precursor that is fed directly into the cells, converted to sialic acid by the sialic acid biosynthetic machinery, and transported to the Golgi apparatus for glycan elaboration. Followed by click reaction with alkyne-containing dye, this system offers a powerful method for imaging the localization, trafficking, and dynamics of glycans, or detection by FACS for quantitative studies. Labeled Glycoproteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-alkyne or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format
- 產品特點
- Simple, fast, does not require lengthy incubation times
- 保存建議
- -20
- 其他
- BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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