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細胞分析試劑盒
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中文名稱

總膠原蛋白檢測試劑盒(無高氯酸鹽)

英文名字
Total Collagen Assay Kit (Perchlorate-Free)
供應商
Biovision
產品貨號
K406-100
產品報價
¥詢價/100Reactions
產品說明書
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購買方式
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產品描述
Collagen is the most abundant protein in mammals, accounting for nearly 30% of body protein content. Collagen is an extracellular scaffolding protein found in skin, tendons, bone, cartilage, ligaments and muscles, amongst other tissues. There are more than twenty different characterized types of collagen, but the fibrilous types I, II and III comprise more than 90% of the total collagen in mammals. Collagen has a triple helical structure, consisting of three ‘preprocollagen’ strands with a repeating Gly-X-Hyp tripeptide sequence, where X is any amino acid and Hyp is hydroxyproline, a post-translationally modified amino acid formed from proline by the enzyme prolyl-4-hydroxylase. In animals, hydroxyproline is found almost entirely in collagen (which contains approximately 12-14% hydroxyproline by mass) and contributes to its structural rigidity and high tensile strength. Hydroxyproline is thus a direct measure of the amount of collagen present in tissues. Many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. BioVision’s Total Collagen Assay Kit II (Perchlorate-Free) is a quick and convenient protocol for detection of small amounts of collagen in a variety of biological samples. The assay is based upon hydrolysis of samples to yield free Hyp, which is oxidized and reacted to give a brightly-colored chromophore with an absorbance peak at 560 nm. The assay employs a proprietary acidic developer solution to accurately measure collagen in hydrolysates without the use of hazardous perchloric acid, obviating the need for any special handling and waste-disposal protocols. The assay can detect a minimum of 0.5 μg collagen per well in a 96-well format and generates colorimetric results that are virtually identical to those of the classical perchloric acid-based method
產品特點
? Simple, fast and convenient assay ? Can detect as low as 0.5 μg of collagen in a variety of samples ? Simple procedure; takes less than ~60 minutes ? Faster than Perchlorate-Based Assay ? PCA-free
保存建議
-20
其他
BioVision的公司總部在美麗的舊金山灣地區,它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品,以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理,艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
注意
該頁面的中文產品信息的翻譯,僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前,請瀏覽說明書確認。
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