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細胞分析試劑盒
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中文名稱

磷脂酶A2活性測定試劑盒(熒光)

英文名字
Phospholipase A2 Activity Assay Kit (Fluorometric)
供應商
Biovision
產品貨號
K400-100
產品報價
¥詢價/100assays
產品說明書
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購買方式
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產品新聞
背景資料
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產品描述
Phospholipase(s) A2 (PLA2s, EC 3.1.1.4) are a family of enzymes that catalyze the hydrolysis an acyl ester bond in the sn-2 position of a glycerophospholipid to release a lysophospholipid and a free fatty acid. Members of the PLA2 superfamily can be divided into three major subtypes based upon their size and cation dependence. Two of the subtypes, the low molecular weight secretory phospholipase A2 enzymes (sPLA2) and the high molecular weight cytosolic enzymes (cPLA2), are characterized by their requirement of Ca2+ for catalytic activity. The 14 kDa sPLA2 enzymes are known to play an important role in the digestion of dietary phospholipids, host defense against bacterial infections as well as production of eicosanoids. The 85 kDa cPLA2 enzymes play an important role in inflammatory disorders by releasing arachidonic acid from membrane phospholipids for production of various eicosanoids. The third subtype, the high molecular weight calcium-independent phospholipases A2 (iPLA2), are intracellular forms of the enzyme that have been recently implicated in functions such as phospholipid remodeling, cell proliferation and fat catabolism. BioVision’s Phospholipase A2 Activity Assay Kit provides a quick, sensitive and simple way for measuring PLA2 activity in various biological samples. In this assay, active PLA2 cleaves a synthetic thiophospholipid, producing a lysothiophospholipid which reacts with a fluorogenic probe to produce a highly fluorescent product detectable in the visible range (Ex/Em= 388/513 nm). The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of PLA2 activity
產品特點
? Simple, rapid & convenient ? The assay can detect total phospholipase A2 (cytosolic and secretory). Activity less than 0.5 mUin various sample types.
保存建議
-20
其他
BioVision的公司總部在美麗的舊金山灣地區,它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品,以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理,艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
注意
該頁面的中文產品信息的翻譯,僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前,請瀏覽說明書確認。
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