- 中文名稱
Interleukin 11, Human (IL-11) BioAssay ELISA Kit
- 英文名字
- Interleukin 11, Human (IL-11) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- I8433-10B
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- This IL-11 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-11. Standards or samples are then added to the appropriate microtiter plate wells and incubated. After washing to remove unbound IL-11 and other components of the sample, biotin-conjugated polyclonal antibody specific to IL-11 is added and incubated. If present, IL-11 will bind and become immobilized by the antibody pre-coated on the wells and then become “sandwiched” by the biotin conjugate. In order to quantitatively determine the amount of IL-11 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits, each having a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-11, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme- substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.In order to measure the concentration of IL-11 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-11 concentration (pg/ml). The concentration of IL-11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: Calibrator Diluent I: 4pg/mlCalibrator Diluent II: 3pg/mlRange: 0-1600pg/mlKit Components:I8433-10B1: Microtiter Plate, 1x96 wellsI8433-10B2: IL-11 Pab (Biotin), 1x7mlI8433-10B3: Avidin (HRP), 1x14ml*I8433-10B4: IL-11 Standard, 2x1 vialI8433-10B5: Calibrator Diluent I, 1x25mlI8433-10B6: Calibrator Diluent II, 1x25mlI8433-10B7: Wash Buffer (20X), 1x60mlI8433-10B8: Substrate A, 1x10mlI8433-10B9: Substrate B, 1x10mlI8433-10B10: Stop Solution, 1x14mlStorage and Stability:Store *I8433-10B4 powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Interleukin 11, Human (IL-11) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Interleukin 11, Human (IL-11) BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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