- 中文名稱
Interleukin 4, Human (IL-4) BioAssay ELISA Kit
- 英文名字
- Interleukin 4, Human (IL-4) BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- I8426-01
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Principle:This IL-4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a polyclonal antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-4 and incubated. IL-4 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be sandwiched by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-4 and other components of the sample. In order to quantitate the amount of IL-4 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-4, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.Sensitivity: Calibrator Diluent I: 6.6pg/mlCalibrator Diluent II: 4.3pg/mlRange: 31.25pg/ml to 1000pg/mlKit Components: I8426-01M: Microtiter Plate 1x96wellsI8426-01N: Mab (Biotin) 1x7mlI8426-01P: Avidin (HRP) 1x14ml*I8426-01Q: Standard 2x1vialI8426-01R: Calibrator Diluent I 1x22ml For serum/plasma testingI8426-01S: Calibrator Diluent II 1x22ml For culture supernatantI8426-01T: Wash Buffer, 20X 1x60mlI8426-01U: Substrate A 1x10mlI8426-01V: Substrate B 1x10mlI8426-01W: Stop Solution 1x14mlSpecificity:This sandwich ELISA recognizes both natural and recombinant human IL-4, exhibiting no detectable crossreactivity with human; SAA, EGF, IL-8, IL-16, MCAF, MCP-3, TNF-g, M-CSF, GM-CSF, TGF-b1, RANTES, FGF, MIP-1a, CRP, EPO, IFN-g.Storage and Stability:Store *I8426-01Q: Standard powder at 4°C, liquid stock at -20°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 產品特點
- 針對Interleukin 4, Human (IL-4) BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Interleukin 4, Human (IL-4) BioAssay ELISA Kit產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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