Plasmid pEGFP-HMGB1 was obtained by inserting the coding sequence for the rat HMGB1 into pEGFP-N1 (Clontech) using the EcoRi and SacII restriction sites (Scaffidi et al. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation. Nature 2002, 418: 191-5). It expresses HMGB1-GFP, which is rat HMGB1 (at the N terminus) fused to GFP (at the C terminus). This protein is fully functional and is functionally equivalent to unmodified HMGB1 (Pallier et al. Association of chromatin proteins HMGB1 and HMGB2 with mitotic chromosomes. Mol Biol Cell 2003, 14: 3414-26).
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HMGBiotech是一家由HMGB領域的領先科學家Marco E. Bianchi創立的生物技術公司。nHMGBiotech是HMGB53質量控制氧化還原變體的唯一生產商。該公司為活躍在這一有前途的領域的其他研究人員和公司提供試劑、服務和想法，并幫助Bianchi實驗室（the Bianchi lab）開發檢測和試劑。
