- 中文名稱
Histamine, BioAssay ELISA Kit
- 英文名字
- Histamine, BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- H5085-20
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- Histamine is a biogenic amine, which is formed by enzymatical decarboxylation from the amino acid histidine. It occurs in the mast cells and basophilic white cells bound to heparin. In the course of a type I allergy, endogenic histamine from the mast cells and basophilic leukocytes is released after antigen binding to membrane-associated IgE, and typical allergical reactions appear.But histamine can also enter the human body via the nutrition and can thus cause pseudoallergic food intolerances. The appearing symptoms are not distinguishable from those of a real allergy. The term 'food allergy' is however only used, if the pathologic symptoms develop in the course of an immunological reaction. Non-immunological reactions to food are defined as food intolerances. Food intolerances, which are caused by increased histamine concentrations, are clinically characterized by rash, diarrhoea, vomiting, nausea, itching, headache and asthma. The extent of the reaction is dependent on the ingested amount of histamine. These reactions can be enhanced by a lack of diaminoxidase.Separated from histamine intolerances, toxic reactions exist, which are caused by very high histamine concentrations. Toxic histamine concentrations, which can cause the so-called scombroid reaction, appear after bacterial degradation of protein-rich food, especially fish. Histamine is found above all in cheese, fish, spinach, smoked products and red wine. The histamine concentrations in different types of food, but also in the same food show frequently great differences. In different types of cheese there can e.g. be found histamine contents between 600 and 1000mg/kg. Histamine ELISA is a quick, economical and sensitive method to detect histamine in wine, fish or cheese. After an appropriate sample preparation, 40 samples can be tested in duplicate within 80 minutes.Intended Use:For the quantitative determination of Histamine in food.Sensitivity: 2ng/mLRecovery (wine, fish, cheese): ~90%Incubation Time: 80 minTest Principle:Histamine, Food, BioAssay ELISA Kit quantitative test is based on the principle of the enzyme linked immunosorbent assay. A histamine conjugate is bound on the surface of a microtiter plate. Samples or standards containing derivatized histamine and an antibody directed against histamine are given into the wells of the microtiter plate. Immobilized and free histamine compete for the antibody binding sites. After 30 minutes incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A peroxidase conjugate directed against the histamine antibody is given into the wells and after another 30 minutes incubation, the plate is washed again. Then a substrate solution is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is inhibited by the addition of a stop solution, and the color turns yellow. The yellow color is measured photometrically at 450nm. The concentration of histamine is indirectly proportional to the color intensity of the test sample.Kit Components:1. Microtiter plate 1x96 wells each, coated with histamine conjugate.2. Standard 4ng/ml 1x4ml each.3. Standard 2ng/ml 1x4ml each.4. Standard 4ng/ml 1x4ml each.5. Standard 10ng/ml 1x4ml each.6. Standard 40ng/ml 1x4ml each.7. Standard 100ng/ml 1x4ml each.8. Reaction Solution: 1x3ml, ready to use.9. Neutralizing Solution: 1x15ml, ready to use.10. Anti-Histamine Antibody (mouse): 1x6ml, ready to use.11. Conjugate (anti-mouse-IgG-HRP): 1x15ml, ready to use.12. Substrate Solution (TMB): 1x15ml, ready to use.13. Stop Solution (0.5 M H2SO4): 1x15ml, ready to use.14. Sample Diluent (PBS): 1x60ml, ready to use.15. Washing Solution 10x (PBS + Tween 20): 1x60ml.Dilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up at 37°C for 15 minutes.Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 產品特點
- 針對Histamine, BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Histamine, BioAssay ELISA Kit產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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