- 中文名稱
Heat Shock Protein 70B’ (HSP70B') BioAssay ELISA Kit
- 英文名字
- Heat Shock Protein 70B’ (HSP70B') BioAssay ELISA Kit
- 供應商
- USBiological
- 產品貨號
- H1834-39
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業?？梢詾槟峁┘皶r的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Hsp70 system is a highly conserved family of ubiquitous proteins found in all prokaryotes and in cellular compartments of eukaryotic organisms (1). Multiple members of this family are involved in protein folding and several other cellular functions (2). Some forms are constitutively expressed in cells, while others are only inducible by metabolic stress (2). Various stressors that induce Hsp70 include heat shock, hypoxia, UV irradiation, CdCl2 and arsenite (1,3). Basal levels of constitutive Hsp70 are found in major intracellular compartments and the inducible Hsp70s are predominantly cytoplasmic and nuclear in distribution (2). There are at least 11 genes for proteins of the human Hsp70 family, which code for a group of highly related proteins ranging from 66 to 78kD (2). The multiple members of this family vary with their basal expression levels and inducibility in response to different stressors (2). The Hsp70 chaperones have two major functional domains (4). The highly conserved NH2-terminal domain has ATPase activity and binds to ADP and ATP very tightly, and COOH-terminal binds to polypeptides (4). Hsp70 is known to bind preferentially to unfolded and partially folded proteins and, prevent their aggregation or misfolding (2). The nomenclature of the different members of Hsp70 family is extensive and is based on cellular distribution and inducibility (2). Human Hsp70B’ is a variant Hsp70 that is more basic than the major Hsp70 and has different stress-induction characteristics (5,6). There have been several reports of Hsp70 variants, but most of them are products of different postranslational modifications (5). Hsp70B’ is a product of a separate gene which is devoid of introns similar to Hsp70 (5). The Hsp70B’ gene has 77% sequence identity to Hsp70 gene and 70% identity to Hsc70 cDNA with highest sequence divergence at the 3’- end (5). Promoter studies have shown Hsp70B’ to be a unique member of the Hsp70 family (5). Unlike Hsp70 which shows basal levels of expression and is induced by heat and various stressors, Hsp70B’ is strictly heat-inducible and shows no basal levels (5). One study reported the use of hsp70B’ as a target gene for studying single nucleotide polymorphisms (SNP) in biopsy samples of human prostate cancer patients (7). However, the use of Hsp70B’ protein as a common biomarker of stress in heat shock protein research is still at its infancy.Number of Samples: 1 standard curve, 39 samples in duplicate or 2 standard curves and 30 samples in duplicate Sensitivity: 60pg/ml Range: 0.156-10ng/mlSample Volume: 100ul/well Incubation Time: <4.5 hours Wavelength Setting: 450nm Materials Included:? Anti-Hsp70B′ Immunoassay Plate, ? 5X Hsp70B, Extraction Reagent, ? Recombinant Hsp70B′ Standard, ? Sample Diluent,? 10X Wash Buffer, ? Anti-Hsp70B′, ? Anti-Hsp70B′ Diluent, ? Anti-Rabbit IgG: HRP Conjugate,? Anti-Rabbit IgG: HRP Conjugate Diluent ? TMB Substrate, ? Acid Stop Solution, ? Instruction Manual Storage and stability:All reagents are stable as supplied at 4°C, except the Recombinant Hsp70B′ Standard, which should be stored at -20°C. For optimum storage, the Recombinant Hsp70B′ Standard should be aliquotted into smaller portions and stored at -20°C. Avoid repeated freeze/thaw cycles.
- 產品特點
- 針對Heat Shock Protein 70B’ (HSP70B') BioAssay ELISA Kit該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Heat Shock Protein 70B’ (HSP70B') BioAssay ELISA Kit產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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